Human TPMT ELISA Kit

Alternative name
Human Thiopurine methyltransferase ELISA Kit;Human TPMTD ELISA Kit;Human thiopurine S-methyltransferase ELISA Kit;Human S-adenosyl-L-methionine:thiopurine S-methyltransferase ELISA Kit;
Catalog
E037985
species
Human
GeneTPMT
SpecificityThis assay has high sensitivity and excellent specificity for detection of Thiopurine Methyltransferase (TPMT). No significant cross-reactivity or interference between Thiopurine Methyltransferase (TPMT) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.65ng/mL.
Intended UseHuman TPMT ELISA Kit allows for the in vitro quantitative determination of TPMT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thiopurine Methyltransferase (TPMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thiopurine Methyltransferase (TPMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thiopurine Methyltransferase (TPMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thiopurine Methyltransferase (TPMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Thiopurine S-methyltransferase Protein information
Uniprot ID TPMT_HUMAN
Uniprot AC O43213; Q5VUK6; Q9UBE6; Q9UBT8; Q9UE62;
UniGene Hs.444319;
GeneID 7172
KEGG hsa:7172;
Human Thiopurine S-methyltransferase Protein SEQUENCE
SEQUENCE 245 AA; 28180 MW; 190E781155B97BB9 CRC64;
MDGTRTSLDI EEYSDTEVQK NQVLTLEEWQ DKWVNGKTAF HQEQGHQLLK
KHLDTFLKGK SGLRVFFPLC GKAVEMKWFA DRGHSVVGVE ISELGIQEFF
TEQNLSYSEE PITEIPGTKV FKSSSGNISL YCCSIFDLPR TNIGKFDMIW
DRGALVAINP GDRKCYADTM FSLLGKKFQY LLCVLSYDPT KHPGPPFYVP
HAEIERLFGK ICNIRCLEKV DAFEERHKSW GIDCLFEKLY LLTEK
UCSC uc003ncm.4; human.;

Category

Human TPMT ELISA Kit

Human Thiopurine methyltransferase ELISA Kit;Human TPMTD ELISA Kit;Human thiopurine S-methyltransferase ELISA Kit;Human S-adenosyl-L-methionine:thiopurine S-methyltransferase ELISA Kit;