Human THBS1 ELISA Kit

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  • Alternative name

    Human TSP ELISA Kit;Human TSP1 ELISA Kit;Human THBS ELISA Kit;Human THBS-1 ELISA Kit;Human TSP-1 ELISA Kit;Human thrombospondin 1 ELISA Kit;Human thrombospondin-1 ELISA Kit;Human thrombospondin-1p180 ELISA Kit;Human thrombospondin-p50 ELISA Kit;

  • Catalog
    E037973
  • species
    Human
  • GeneTHBS1
  • Other Species Mouse Thbs1 ELISA KitBovine THBS1 ELISA Kit
  • SpecificityNatural and recombinant human THBS1
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 50pg/ml
  • Intended UseHuman THBS1 ELISA Kit allows for the in vitro quantitative determination of THBS1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human THBS1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for THBS1 has been precoated onto 96-well plates. Standards(NSO, N19-P1170) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for THBS1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human THBS1 amount of sample captured in plate. Background: Thrombospondin 1, also known as THBS1, is a protein that in humans in encoded by the THBS1 gene. Thrombospondin 1 is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. By in situ hybridization, the THBS1 gene was mapped to human 15q15 and the cognate gene to mouse chromosome 2 (region F) and was localized to 15q11-qter by Southern analysis of human-rodent somatic cell hybrids. Thrombospondin I is a multimodular secreted protein that associates with the extracellular matrix and possesses a variety of biologic functions, including a potent antiangiogenic activity. Other thrombospondin genes include thrombospondins II, III, and IV.
  • Human Thrombospondin-1 Protein information
  • Uniprot ID TSP1_HUMAN
  • Uniprot AC P07996; A8K6H4; B4E3J7; B9EGH6; Q15667; Q59E99;
  • UniGene Hs.164226; Hs.732539;
  • GeneID 7057
  • KEGG hsa:7057;
  • Human Thrombospondin-1 Protein SEQUENCE
  • SEQUENCE 1170 AA; 129383 MW; 74749B2418E0943B CRC64;

    MGLAWGLGVL FLMHVCGTNR IPESGGDNSV FDIFELTGAA RKGSGRRLVK

    GPDPSSPAFR IEDANLIPPV PDDKFQDLVD AVRAEKGFLL LASLRQMKKT

    RGTLLALERK DHSGQVFSVV SNGKAGTLDL SLTVQGKQHV VSVEEALLAT

    GQWKSITLFV QEDRAQLYID CEKMENAELD VPIQSVFTRD LASIARLRIA

    KGGVNDNFQG VLQNVRFVFG TTPEDILRNK GCSSSTSVLL TLDNNVVNGS

    SPAIRTNYIG HKTKDLQAIC GISCDELSSM VLELRGLRTI VTTLQDSIRK

    VTEENKELAN ELRRPPLCYH NGVQYRNNEE WTVDSCTECH CQNSVTICKK

    VSCPIMPCSN ATVPDGECCP RCWPSDSADD GWSPWSEWTS CSTSCGNGIQ

    QRGRSCDSLN NRCEGSSVQT RTCHIQECDK RFKQDGGWSH WSPWSSCSVT

    CGDGVITRIR LCNSPSPQMN GKPCEGEARE TKACKKDACP INGGWGPWSP

    WDICSVTCGG GVQKRSRLCN NPTPQFGGKD CVGDVTENQI CNKQDCPIDG

    CLSNPCFAGV KCTSYPDGSW KCGACPPGYS GNGIQCTDVD ECKEVPDACF

    NHNGEHRCEN TDPGYNCLPC PPRFTGSQPF GQGVEHATAN KQVCKPRNPC

    TDGTHDCNKN AKCNYLGHYS DPMYRCECKP GYAGNGIICG EDTDLDGWPN

    ENLVCVANAT YHCKKDNCPN LPNSGQEDYD KDGIGDACDD DDDNDKIPDD

    RDNCPFHYNP AQYDYDRDDV GDRCDNCPYN HNPDQADTDN NGEGDACAAD

    IDGDGILNER DNCQYVYNVD QRDTDMDGVG DQCDNCPLEH NPDQLDSDSD

    RIGDTCDNNQ DIDEDGHQNN LDNCPYVPNA NQADHDKDGK GDACDHDDDN

    DGIPDDKDNC RLVPNPDQKD SDGDGRGDAC KDDFDHDSVP DIDDICPENV

    DISETDFRRF QMIPLDPKGT SQNDPNWVVR HQGKELVQTV NCDPGLAVGY

    DEFNAVDFSG TFFINTERDD DYAGFVFGYQ SSSRFYVVMW KQVTQSYWDT

    NPTRAQGYSG LSVKVVNSTT GPGEHLRNAL WHTGNTPGQV RTLWHDPRHI

    GWKDFTAYRW RLSHRPKTGF IRVVMYEGKK IMADSGPIYD KTYAGGRLGL

    FVFSQEMVFF SDLKYECRDP

  • UCSC uc001zkh.4; human. [P07996-1];


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