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Catalog
E046329
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species
Mouse
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.195 mU/ml
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Intended UseMouse CCTGF ELISA Kit allows for the in vitro quantitative determination of , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C-terminal peptide of bone-specific alkaline phosphatase has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C-terminal peptide of bone-specific alkaline phosphatase present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C-terminal peptide of bone-specific alkaline phosphatase is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C-terminal peptide of bone-specific alkaline phosphatase bound in the initial step. The color development is stopped and the intensity of the color is measured.
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