Human TEF ELISA Kit

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  • Alternative name

    Human KIAA1655 ELISA Kit;Human TEF, PAR bZIP transcription factor ELISA Kit;Human thyrotroph embryonic factor ELISA Kit;Human thyrotrophic embryonic factor ELISA Kit;

  • Catalog
    E037650
  • species
    Human
  • GeneTEF
  • Other Species Mouse Tef ELISA KitChicken TEF ELISA KitRat Tef ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human TEF. No significant cross-reactivity or interference between Human TEF and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human TEF and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman TEF ELISA Kit allows for the in vitro quantitative determination of TEF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TEF in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TEF. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human TEF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TEF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human TEF. You can calculate the concentration of Human TEF in the samples by comparing the OD of the samples to the standard curve.
  • Human Thyrotroph embryonic factor Protein information
  • Uniprot ID TEF_HUMAN
  • Uniprot AC Q10587; B0QYS8; B2RC22; Q15729; Q7Z3J7; Q8IU94; Q96TG4;
  • UniGene Hs.181159;
  • GeneID 7008
  • KEGG hsa:7008;
  • Human Thyrotroph embryonic factor Protein SEQUENCE
  • SEQUENCE 303 AA; 33248 MW; 4A87B7BFA7248C6F CRC64;

    MSDAGGGKKP PVDPQAGPGP GPGRAAGERG LSGSFPLVLK KLMENPPREA

    RLDKEKGKEK LEEDEAAAAS TMAVSASLMP PIWDKTIPYD GESFHLEYMD

    LDEFLLENGI PASPTHLAHN LLLPVAELEG KESASSSTAS PPSSSTAIFQ

    PSETVSSTES SLEKERETPS PIDPNCVEVD VNFNPDPADL VLSSVPGGEL

    FNPRKHKFAE EDLKPQPMIK KAKKVFVPDE QKDEKYWTRR KKNNVAAKRS

    RDARRLKENQ ITIRAAFLEK ENTALRTEVA ELRKEVGKCK TIVSKYETKY

    GPL

  • UCSC uc003azx.5; human. [Q10587-1];


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