Human AFMID ELISA Kit

Alternative name
Human KFA ELISA Kit;Human KFase ELISA Kit;Human arylformamidase ELISA Kit;Human N-formylkynurenine formamidase ELISA Kit;Human FKF ELISA Kit;Human KF ELISA Kit;Human kynurenine formamidase ELISA Kit;Human probable arylformamidase ELISA Kit;
Catalog
E003762
species
Human
GeneAFMID
Standard Curve
Other Species Mouse Afmid ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Arylformamidase (AFMID). No significant cross-reactivity or interference between Arylformamidase (AFMID) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.57ng/mL.
Intended UseHuman AFMID ELISA Kit allows for the in vitro quantitative determination of AFMID , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arylformamidase (AFMID). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arylformamidase (AFMID). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arylformamidase (AFMID), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arylformamidase (AFMID) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Kynurenine formamidase Protein information
Uniprot ID KFA_HUMAN
Uniprot AC Q63HM1; A2RUB3;
UniGene Hs.558614;
GeneID 125061
KEGG hsa:125061;
Human Kynurenine formamidase Protein SEQUENCE
SEQUENCE 303 AA; 33992 MW; 6E329218366C027D CRC64;
MMDVSGVGFP SKVPWKKMSA EELENQYCPS RWVVRLGAEE ALRTYSQIGI
EATTRARATR KSLLHVPYGD GEGEKVDIYF PDESSEALPF FLFFHGGYWQ
SGSKDESAFM VHPLTAQGVA VVIVAYGIAP KGTLDHMVDQ VTRSVAFVQK
RYPSNKGIYL CGHSAGAHLA AMMLLADWTK HGVTPNLRGF FLVSGVFDLE
PIVYTSQNVA LQLTLEDAQR NSPQLKVAQA QPVDPTCRVL VVVGQFDSPE
FHRQSWEFYQ TLCQGEWKAS FEELHDVDHF EIVENLTQKD NVLTQIILKT
IFQ
UCSC uc002juz.4; human. [Q63HM1-1];

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anti-AFMID antibody

Category

Human AFMID ELISA Kit

Human KFA ELISA Kit;Human KFase ELISA Kit;Human arylformamidase ELISA Kit;Human N-formylkynurenine formamidase ELISA Kit;Human FKF ELISA Kit;Human KF ELISA Kit;Human kynurenine formamidase ELISA Kit;Human probable arylformamidase ELISA Kit;