Human AKT2 ELISA Kit

Alternative name
Human protein kinase Akt-2 ELISA Kit;Human protein kinase B beta ELISA Kit;Human PKB beta ELISA Kit;Human RAC-PK-beta ELISA Kit;Human HIHGHH ELISA Kit;Human PKBB ELISA Kit;Human PKBBETA ELISA Kit;Human PRKBB ELISA Kit;Human RAC-BETA ELISA Kit;Human AKT serine/threonine kinase 2 ELISA Kit;Human RAC-beta serine/threonine-protein kinase ELISA Kit;Human murine thymoma viral (v-akt) homolog-2 ELISA Kit;Human putative v-akt murine thymoma viral oncoprotein 2 ELISA Kit;Human rac protein kinase beta ELISA Kit;Human v-akt murine thymoma viral oncogene homolog 2 ELISA Kit;
Catalog
E032727
species
Human
GeneAKT2
Standard Curve
Other Species Mouse Akt2 ELISA Kit Rat Akt2 ELISA Kit
SpecificityThis assay recognizes recombinant and natural humanRAC-beta serine/threonine-protein kinase. No significant cross-reactivity or interference was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman AKT2 ELISA Kit allows for the in vitro quantitative determination of AKT2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: This immunoassay kit allows for the in vitro quantitative determination ofhumanRAC-beta serine/threonine-protein kinaseconcentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids. Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAC-beta serine/threonine-protein kinase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forRAC-beta serine/threonine-protein kinase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain RAC-beta serine/threonine-protein kinase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of RAC-beta serine/threonine-protein kinase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human RAC-beta serine/threonine-protein kinase Protein information
Uniprot ID AKT2_HUMAN
Uniprot AC P31751; B2RBD8; Q05BV0; Q0VAN0; Q0VAN1; Q68GC0;
UniGene Hs.631535;
GeneID 208
KEGG hsa:208;
Human RAC-beta serine/threonine-protein kinase Protein SEQUENCE
SEQUENCE 481 AA; 55769 MW; B18C87A7246BFB24 CRC64;
MNEVSVIKEG WLHKRGEYIK TWRPRYFLLK SDGSFIGYKE RPEAPDQTLP
PLNNFSVAEC QLMKTERPRP NTFVIRCLQW TTVIERTFHV DSPDEREEWM
RAIQMVANSL KQRAPGEDPM DYKCGSPSDS STTEEMEVAV SKARAKVTMN
DFDYLKLLGK GTFGKVILVR EKATGRYYAM KILRKEVIIA KDEVAHTVTE
SRVLQNTRHP FLTALKYAFQ THDRLCFVME YANGGELFFH LSRERVFTEE
RARFYGAEIV SALEYLHSRD VVYRDIKLEN LMLDKDGHIK ITDFGLCKEG
ISDGATMKTF CGTPEYLAPE VLEDNDYGRA VDWWGLGVVM YEMMCGRLPF
YNQDHERLFE LILMEEIRFP RTLSPEAKSL LAGLLKKDPK QRLGGGPSDA
KEVMEHRFFL SINWQDVVQK KLLPPFKPQV TSEVDTRYFD DEFTAQSITI
TPPDRYDSLG LLELDQRTHF PQFSYSASIR E
UCSC uc002onf.3; human. [P31751-1];

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Human AKT2 ELISA Kit