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Alternative name
Superoxide dismutase [Cu-Zn] 4AP ELISA KIT; SODCC.2 ELISA KIT; SOD4AP ELISA KIT
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Catalog
E036945
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species
Human
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GeneSOD4
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Superoxide Dismutase, Copper Chaperone (SOD4).
No significant cross-reactivity or interference between Superoxide Dismutase, Copper Chaperone (SOD4) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.117ng/mL.
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Intended UseHuman SOD4 ELISA Kit allows for the in vitro quantitative determination of SOD4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Superoxide Dismutase, Copper Chaperone (SOD4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Superoxide Dismutase, Copper Chaperone (SOD4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Superoxide Dismutase, Copper Chaperone (SOD4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Superoxide Dismutase, Copper Chaperone (SOD4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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