Human APCS ELISA Kit

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  • Alternative name

    Human SAP ELISA Kit;Human 9.5S alpha-1-glycoprotein ELISA Kit;Human PTX2 ELISA Kit;Human HEL-S-92n ELISA Kit;Human amyloid P component, serum ELISA Kit;Human serum amyloid P-component ELISA Kit;Human epididymis secretory sperm binding protein Li 92n ELISA Kit;Human pentaxin-related ELISA Kit;Human pentraxin-2 ELISA Kit;Human pentraxin-related ELISA Kit;

  • Catalog
    E001925
  • species
    Human
  • GeneAPCS
  • Other Species Mouse Apcs ELISA KitBovine APCS ELISA KitPorcine APCS ELISA KitRat Apcs ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human APCS. No significant cross-reactivity or interference between Human APCS and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.95 ng/ml
  • Intended UseHuman APCS ELISA Kit allows for the in vitro quantitative determination of APCS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Introduction: Amyloid P component, serum (SAP) is the identical serum form of amyloid P component (AP), a 25kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". AP makes up 14% of the dry mass of amyloid deposits and is thought to be an important contributor to the pathogenesis of a related group of diseases called the Amyloidoses. SAP is a member of the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. Human SAP has 51% sequence homology with C-reactive protein (CRP) Both SAP and CRP are evolutionary conserved in all vertebrates and also found in distant invertebrates such as the horseshoe crab. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SAP. Standards or samples are then added to the appropriate microtiter plate wells with a 3 biotin-conjugated antibody preparation specific for SAP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain SAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of SAP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Serum amyloid P-component(1-203) Protein information
  • Uniprot ID SAMP_HUMAN
  • Uniprot AC P02743;
  • UniGene Hs.507080;
  • GeneID 325
  • KEGG hsa:325;
  • Human Serum amyloid P-component(1-203) Protein SEQUENCE
  • SEQUENCE 223 AA; 25387 MW; 6C88A515FE88B393 CRC64;

    MNKPLLWISV LTSLLEAFAH TDLSGKVFVF PRESVTDHVN LITPLEKPLQ

    NFTLCFRAYS DLSRAYSLFS YNTQGRDNEL LVYKERVGEY SLYIGRHKVT

    SKVIEKFPAP VHICVSWESS SGIAEFWING TPLVKKGLRQ GYFVEAQPKI

    VLGQEQDSYG GKFDRSQSFV GEIGDLYMWD SVLPPENILS AYQGTPLPAN

    ILDWQALNYE IRGYVIIKPL VWV





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