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  • Alternative name

    RING finger protein 112 ELISA KIT; Brain finger protein ELISA KIT; Zinc finger protein 179RNF112 ELISA KIT; BFP ELISA KIT; ZNF179 ELISA KIT

  • Catalog
  • species
  • GeneBFP
  • Standard CurveMouse BFP ELISA Kit
  • Other Species Human BFP ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Brain Finger Protein (BFP). No significant cross-reactivity or interference between Brain Finger Protein (BFP) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.066ng/mL.
  • Intended UseMouse BFP ELISA Kit allows for the in vitro quantitative determination of BFP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Brain Finger Protein (BFP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Brain Finger Protein (BFP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Brain Finger Protein (BFP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Brain Finger Protein (BFP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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