Human S1PR3 ELISA Kit

Alternative name
Human S1P receptor 3 ELISA Kit;Human S1P3 ELISA Kit;Human endothelial differentiation G-protein coupled receptor 3 ELISA Kit;Human sphingosine 1-phosphate receptor Edg-3 ELISA Kit;Human S1P receptor Edg-3 ELISA Kit;Human EDG3 ELISA Kit;Human EDG-3 ELISA Kit;Human LPB3 ELISA Kit;Human sphingosine-1-phosphate receptor 3 ELISA Kit;Human sphingosine 1-phosphate receptor 3 ELISA Kit;Human G protein-coupled receptor, endothelial differentiation gene-3 ELISA Kit;Human S1P receptor EDG3 ELISA Kit;Human endothelial differentiation, sphingolipid G-protein-coupled receptor, 3 ELISA Kit;
Catalog
E036437
species
Human
GeneS1PR3
SpecificityThis assay has high sensitivity and excellent specificity for detection of Sphingosine 1 Phosphate Receptor 3 (S1PR3). No significant cross-reactivity or interference between Sphingosine 1 Phosphate Receptor 3 (S1PR3) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.65ng/mL.
Intended UseHuman S1PR3 ELISA Kit allows for the in vitro quantitative determination of S1PR3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine 1 Phosphate Receptor 3 (S1PR3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sphingosine 1 Phosphate Receptor 3 (S1PR3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sphingosine 1 Phosphate Receptor 3 (S1PR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sphingosine 1 Phosphate Receptor 3 (S1PR3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Sphingosine 1-phosphate receptor 3 Protein information
Uniprot ID S1PR3_HUMAN
Uniprot AC Q99500; Q5SQD8; Q7Z5I2;
UniGene Hs.585118;
GeneID 1903
KEGG hsa:1903;
Human Sphingosine 1-phosphate receptor 3 Protein SEQUENCE
SEQUENCE 378 AA; 42250 MW; 79A00300968F439F CRC64;
MATALPPRLQ PVRGNETLRE HYQYVGKLAG RLKEASEGST LTTVLFLVIC
SFIVLENLMV LIAIWKNNKF HNRMYFFIGN LALCDLLAGI AYKVNILMSG
KKTFSLSPTV WFLREGSMFV ALGASTCSLL AIAIERHLTM IKMRPYDANK
RHRVFLLIGM CWLIAFTLGA LPILGWNCLH NLPDCSTILP LYSKKYIAFC
ISIFTAILVT IVILYARIYF LVKSSSRKVA NHNNSERSMA LLRTVVIVVS
VFIACWSPLF ILFLIDVACR VQACPILFKA QWFIVLAVLN SAMNPVIYTL
ASKEMRRAFF RLVCNCLVRG RGARASPIQP ALDPSRSKSS SSNNSSHSPK
VKEDLPHTAP SSCIMDKNAA LQNGIFCN
UCSC uc004aqe.5; human.;

Category

Human S1PR3 ELISA Kit