Human CRLF1 ELISA Kit

Alternative name
Human cytokine-like factor 1 ELISA Kit;Human CLF-1 ELISA Kit;Human zcytor5 ELISA Kit;Human CISS ELISA Kit;Human CISS1 ELISA Kit;Human CLF ELISA Kit;Human NR6 ELISA Kit;Human cytokine receptor like factor 1 ELISA Kit;Human cytokine receptor-like factor 1 ELISA Kit;Human class I cytokine receptor ELISA Kit;Human cytokine type 1 receptor CRLP-1 ELISA Kit;
Catalog
E011196
species
Human
GeneCRLF1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cytokine Receptor Like Factor 1 (CRLF1). No significant cross-reactivity or interference between Cytokine Receptor Like Factor 1 (CRLF1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.053ng/mL.
Intended UseHuman CRLF1 ELISA Kit allows for the in vitro quantitative determination of CRLF1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytokine Receptor Like Factor 1 (CRLF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytokine Receptor Like Factor 1 (CRLF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytokine Receptor Like Factor 1 (CRLF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytokine Receptor Like Factor 1 (CRLF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Cytokine receptor-like factor 1 Protein information
Uniprot ID CRLF1_HUMAN
Uniprot AC O75462; Q9UHH5;
UniGene Hs.114948;
GeneID 9244
KEGG hsa:9244;
Human Cytokine receptor-like factor 1 Protein SEQUENCE
SEQUENCE 422 AA; 46302 MW; AD9DEFCB01B84228 CRC64;
MPAGRRGPAA QSARRPPPLL PLLLLLCVLG APRAGSGAHT AVISPQDPTL
LIGSSLLATC SVHGDPPGAT AEGLYWTLNG RRLPPELSRV LNASTLALAL
ANLNGSRQRS GDNLVCHARD GSILAGSCLY VGLPPEKPVN ISCWSKNMKD
LTCRWTPGAH GETFLHTNYS LKYKLRWYGQ DNTCEEYHTV GPHSCHIPKD
LALFTPYEIW VEATNRLGSA RSDVLTLDIL DVVTTDPPPD VHVSRVGGLE
DQLSVRWVSP PALKDFLFQA KYQIRYRVED SVDWKVVDDV SNQTSCRLAG
LKPGTVYFVQ VRCNPFGIYG SKKAGIWSEW SHPTAASTPR SERPGPGGGA
CEPRGGEPSS GPVRRELKQF LGWLKKHAYC SNLSFRLYDQ WRAWMQKSHK
TRNQDEGILP SGRRGTARGP AR
UCSC uc010ebt.3; human.;

Category

Human CRLF1 ELISA Kit