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Human SEMA4D ELISA Kit

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  • Alternative name

    Human A8 ELISA Kit;Human BB18 ELISA Kit;Human GR3 ELISA Kit;Human C9orf164 ELISA Kit;Human CD100 ELISA Kit;Human SEMAJ ELISA Kit;Human M-sema-G ELISA Kit;Human coll-4 ELISA Kit;Human semaphorin 4D ELISA Kit;Human semaphorin-4D ELISA Kit;Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D ELISA Kit;Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D ELISA Kit;
  • Catalog
    E034604
  • species
    Human
  • GeneSEMA4D
  • Standard CurveHuman SEMA4D ELISA Kit
  • Other Species Mouse Sema4d ELISA KitMouse SEMA4D/CD100 ELISA KitChicken SEMA4D ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Semaphorin 4D (SEMA4D). No significant cross-reactivity or interference between Semaphorin 4D (SEMA4D) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.61ng/mL.
  • Intended UseHuman SEMA4D ELISA Kit allows for the in vitro quantitative determination of SEMA4D , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 4D (SEMA4D). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 4D (SEMA4D). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 4D (SEMA4D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Semaphorin 4D (SEMA4D) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Semaphorin-4D Protein information
  • Uniprot ID SEM4D_HUMAN
  • Uniprot AC Q92854; B2RPM6; Q7Z5S4; Q8N8B0;
  • UniGene Hs.494406;
  • GeneID 10507
  • KEGG hsa:10507;
  • Human Semaphorin-4D Protein SEQUENCE

  • SEQUENCE 862 AA; 96150 MW; 7B18EFEA98789371 CRC64;

    MRMCTPIRGL LMALAVMFGT AMAFAPIPRI TWEHREVHLV QFHEPDIYNY

    SALLLSEDKD TLYIGAREAV FAVNALNISE KQHEVYWKVS EDKKAKCAEK

    GKSKQTECLN YIRVLQPLSA TSLYVCGTNA FQPACDHLNL TSFKFLGKNE

    DGKGRCPFDP AHSYTSVMVD GELYSGTSYN FLGSEPIISR NSSHSPLRTE

    YAIPWLNEPS FVFADVIRKS PDSPDGEDDR VYFFFTEVSV EYEFVFRVLI

    PRIARVCKGD QGGLRTLQKK WTSFLKARLI CSRPDSGLVF NVLRDVFVLR

    SPGLKVPVFY ALFTPQLNNV GLSAVCAYNL STAEEVFSHG KYMQSTTVEQ

    SHTKWVRYNG PVPKPRPGAC IDSEARAANY TSSLNLPDKT LQFVKDHPLM

    DDSVTPIDNR PRLIKKDVNY TQIVVDRTQA LDGTVYDVMF VSTDRGALHK

    AISLEHAVHI IEETQLFQDF EPVQTLLLSS KKGNRFVYAG SNSGVVQAPL

    AFCGKHGTCE DCVLARDPYC AWSPPTATCV ALHQTESPSR GLIQEMSGDA

    SVCPDKSKGS YRQHFFKHGG TAELKCSQKS NLARVFWKFQ NGVLKAESPK

    YGLMGRKNLL IFNLSEGDSG VYQCLSEERV KNKTVFQVVA KHVLEVKVVP

    KPVVAPTLSV VQTEGSRIAT KVLVASTQGS SPPTPAVQAT SSGAITLPPK

    PAPTGTSCEP KIVINTVPQL HSEKTMYLKS SDNRLLMSLF LFFFVLFLCL

    FFYNCYKGYL PRQCLKFRSA LLIGKKKPKS DFCDREQSLK ETLVEPGSFS

    QQNGEHPKPA LDTGYETEQD TITSKVPTDR EDSQRIDDLS ARDKPFDVKC

    ELKFADSDAD GD

  • UCSC uc004aqo.2; human. [Q92854-1];


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