Human ACTN1 ELISA Kit

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  • Alternative name

    Human alpha-actinin cytoskeletal isoform ELISA Kit;Human F-actin cross-linking protein ELISA Kit;Human non-muscle alpha-actinin-1 ELISA Kit;Human BDPLT15 ELISA Kit;Human actinin alpha 1 ELISA Kit;Human alpha-actinin-1 ELISA Kit;Human actinin 1 smooth muscle ELISA Kit;

  • Catalog
    E000454
  • species
    Human
  • GeneACTN1
  • Other Species Mouse Actn1 ELISA KitBovine ACTN1 ELISA KitChicken ACTN1 ELISA KitMonkey ACTN1 ELISA KitRat Actn1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human ACTN-1. No significant cross-reactivity or interference between human ACTN-1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human ACTN-1 is typically less than 0.039 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m
  • Intended UseHuman ACTN1 ELISA Kit allows for the in vitro quantitative determination of ACTN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ACTN-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACTN-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACTN-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACTN-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Alpha-actinin-1 Protein information
  • Uniprot ID ACTN1_HUMAN
  • Uniprot AC P12814; B3V8S3; B4DHH3; B7TY16; Q1HE25; Q9BTN1;
  • UniGene Hs.235750; Hs.509765;
  • GeneID 87
  • KEGG hsa:87;
  • Human Alpha-actinin-1 Protein SEQUENCE
  • SEQUENCE 892 AA; 103058 MW; 6DA3E4D1A0289519 CRC64;

    MDHYDSQQTN DYMQPEEDWD RDLLLDPAWE KQQRKTFTAW CNSHLRKAGT

    QIENIEEDFR DGLKLMLLLE VISGERLAKP ERGKMRVHKI SNVNKALDFI

    ASKGVKLVSI GAEEIVDGNV KMTLGMIWTI ILRFAIQDIS VEETSAKEGL

    LLWCQRKTAP YKNVNIQNFH ISWKDGLGFC ALIHRHRPEL IDYGKLRKDD

    PLTNLNTAFD VAEKYLDIPK MLDAEDIVGT ARPDEKAIMT YVSSFYHAFS

    GAQKAETAAN RICKVLAVNQ ENEQLMEDYE KLASDLLEWI RRTIPWLENR

    VPENTMHAMQ QKLEDFRDYR RLHKPPKVQE KCQLEINFNT LQTKLRLSNR

    PAFMPSEGRM VSDINNAWGC LEQVEKGYEE WLLNEIRRLE RLDHLAEKFR

    QKASIHEAWT DGKEAMLRQK DYETATLSEI KALLKKHEAF ESDLAAHQDR

    VEQIAAIAQE LNELDYYDSP SVNARCQKIC DQWDNLGALT QKRREALERT

    EKLLETIDQL YLEYAKRAAP FNNWMEGAME DLQDTFIVHT IEEIQGLTTA

    HEQFKATLPD ADKERLAILG IHNEVSKIVQ TYHVNMAGTN PYTTITPQEI

    NGKWDHVRQL VPRRDQALTE EHARQQHNER LRKQFGAQAN VIGPWIQTKM

    EEIGRISIEM HGTLEDQLSH LRQYEKSIVN YKPKIDQLEG DHQLIQEALI

    FDNKHTNYTM EHIRVGWEQL LTTIARTINE VENQILTRDA KGISQEQMNE

    FRASFNHFDR DHSGTLGPEE FKACLISLGY DIGNDPQGEA EFARIMSIVD

    PNRLGVVTFQ AFIDFMSRET ADTDTADQVM ASFKILAGDK NYITMDELRR

    ELPPDQAEYC IARMAPYTGP DSVPGALDYM SFSTALYGES DL

  • UCSC uc001xkl.4; human. [P12814-1];




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