Human ACTN1 ELISA Kit

Alternative name
Human alpha-actinin cytoskeletal isoform ELISA Kit;Human F-actin cross-linking protein ELISA Kit;Human non-muscle alpha-actinin-1 ELISA Kit;Human BDPLT15 ELISA Kit;Human actinin alpha 1 ELISA Kit;Human alpha-actinin-1 ELISA Kit;Human actinin 1 smooth muscle ELISA Kit;
Catalog
E000454
species
Human
GeneACTN1
Other Species Mouse Actn1 ELISA Kit Bovine ACTN1 ELISA Kit Chicken ACTN1 ELISA Kit Monkey ACTN1 ELISA Kit Rat Actn1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of human ACTN-1. No significant cross-reactivity or interference between human ACTN-1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human ACTN-1 is typically less than 0.039 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m
Intended UseHuman ACTN1 ELISA Kit allows for the in vitro quantitative determination of ACTN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ACTN-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACTN-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACTN-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACTN-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Alpha-actinin-1 Protein information
Uniprot ID ACTN1_HUMAN
Uniprot AC P12814; B3V8S3; B4DHH3; B7TY16; Q1HE25; Q9BTN1;
UniGene Hs.235750; Hs.509765;
GeneID 87
KEGG hsa:87;
Human Alpha-actinin-1 Protein SEQUENCE
SEQUENCE 892 AA; 103058 MW; 6DA3E4D1A0289519 CRC64;
MDHYDSQQTN DYMQPEEDWD RDLLLDPAWE KQQRKTFTAW CNSHLRKAGT
QIENIEEDFR DGLKLMLLLE VISGERLAKP ERGKMRVHKI SNVNKALDFI
ASKGVKLVSI GAEEIVDGNV KMTLGMIWTI ILRFAIQDIS VEETSAKEGL
LLWCQRKTAP YKNVNIQNFH ISWKDGLGFC ALIHRHRPEL IDYGKLRKDD
PLTNLNTAFD VAEKYLDIPK MLDAEDIVGT ARPDEKAIMT YVSSFYHAFS
GAQKAETAAN RICKVLAVNQ ENEQLMEDYE KLASDLLEWI RRTIPWLENR
VPENTMHAMQ QKLEDFRDYR RLHKPPKVQE KCQLEINFNT LQTKLRLSNR
PAFMPSEGRM VSDINNAWGC LEQVEKGYEE WLLNEIRRLE RLDHLAEKFR
QKASIHEAWT DGKEAMLRQK DYETATLSEI KALLKKHEAF ESDLAAHQDR
VEQIAAIAQE LNELDYYDSP SVNARCQKIC DQWDNLGALT QKRREALERT
EKLLETIDQL YLEYAKRAAP FNNWMEGAME DLQDTFIVHT IEEIQGLTTA
HEQFKATLPD ADKERLAILG IHNEVSKIVQ TYHVNMAGTN PYTTITPQEI
NGKWDHVRQL VPRRDQALTE EHARQQHNER LRKQFGAQAN VIGPWIQTKM
EEIGRISIEM HGTLEDQLSH LRQYEKSIVN YKPKIDQLEG DHQLIQEALI
FDNKHTNYTM EHIRVGWEQL LTTIARTINE VENQILTRDA KGISQEQMNE
FRASFNHFDR DHSGTLGPEE FKACLISLGY DIGNDPQGEA EFARIMSIVD
PNRLGVVTFQ AFIDFMSRET ADTDTADQVM ASFKILAGDK NYITMDELRR
ELPPDQAEYC IARMAPYTGP DSVPGALDYM SFSTALYGES DL
UCSC uc001xkl.4; human. [P12814-1];

Category

Human ACTN1 ELISA Kit

Human alpha-actinin cytoskeletal isoform ELISA Kit;Human F-actin cross-linking protein ELISA Kit;Human non-muscle alpha-actinin-1 ELISA Kit;Human BDPLT15 ELISA Kit;Human actinin alpha 1 ELISA Kit;Human alpha-actinin-1 ELISA Kit;Human actinin 1 smooth muscle ELISA Kit;