SpecificityThis assay has high sensitivity and excellent specificity for detection of SEMA-3. No significant cross-reactivity or interference between SEMA-3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SEMA-3 and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman SEMA3 ELISA Kit allows for the in vitro quantitative determination of SEMA3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntended Uses: This SEMA-3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SEMA-3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||SEMA-3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SEMA-3. Standards or samples are then added to the microtiter plate wells and SEMA-3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SEMA-3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SEMA-3 are added to each well to "sandwich" the SEMA-3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SEMA-3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SEMA-3 concentration in each sample is interpolated from this standard curve.