Human SCG3 ELISA Kit

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  • Alternative name

    Human secretogranin III ELISA Kit;Human SGIII ELISA Kit;Human secretogranin-3 ELISA Kit;

  • Catalog
    E034482
  • species
    Human
  • GeneSCG3
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Secretogranin III (SCG3). No significant cross-reactivity or interference between Secretogranin III (SCG3) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.063ng/mL.
  • Intended UseHuman SCG3 ELISA Kit allows for the in vitro quantitative determination of SCG3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secretogranin III (SCG3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secretogranin III (SCG3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secretogranin III (SCG3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secretogranin III (SCG3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Secretogranin-3 Protein information
  • Uniprot ID SCG3_HUMAN
  • Uniprot AC Q8WXD2; A8K2B0; B3KQP6; B4DK99; F5H3R8; Q96C83; Q96GE8; Q9Y6G7;
  • UniGene Hs.232618;
  • GeneID 29106
  • KEGG hsa:29106;
  • Human Secretogranin-3 Protein SEQUENCE
  • SEQUENCE 468 AA; 53005 MW; 5E6BBDDFFF3B8D82 CRC64;

    MGFLGTGTWI LVLVLPIQAF PKPGGSQDKS LHNRELSAER PLNEQIAEAE

    EDKIKKTYPP ENKPGQSNYS FVDNLNLLKA ITEKEKIEKE RQSIRSSPLD

    NKLNVEDVDS TKNRKLIDDY DSTKSGLDHK FQDDPDGLHQ LDGTPLTAED

    IVHKIAARIY EENDRAVFDK IVSKLLNLGL ITESQAHTLE DEVAEVLQKL

    ISKEANNYEE DPNKPTSWTE NQAGKIPEKV TPMAAIQDGL AKGENDETVS

    NTLTLTNGLE RRTKTYSEDN FEELQYFPNF YALLKSIDSE KEAKEKETLI

    TIMKTLIDFV KMMVKYGTIS PEEGVSYLEN LDEMIALQTK NKLEKNATDN

    ISKLFPAPSE KSHEETDSTK EEAAKMEKEY GSLKDSTKDD NSNPGGKTDE

    PKGKTEAYLE AIRKNIEWLK KHDKKGNKED YDLSKMRDFI NKQADAYVEK

    GILDKEEAEA IKRIYSSL

  • UCSC uc002abh.4; human. [Q8WXD2-1];



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