Human CES2 ELISA Kit

Alternative name
Human carboxylesterase 2 ELISA Kit;Human CE-2 ELISA Kit;Human hCE-2 ELISA Kit;Human methylumbelliferyl-acetate deacetylase 2 ELISA Kit;Human iCE ELISA Kit;Human CES2A1 ELISA Kit;Human PCE-2 ELISA Kit;Human cocaine esterase ELISA Kit;Human carboxylesterase 2 (intestine, liver) ELISA Kit;Human intestinal carboxylesterase; liver carboxylesterase-2 ELISA Kit;
Catalog
E009899
species
Human
GeneCES2
SpecificityThis assay has high sensitivity and excellent specificity for detection of CES2. No significant cross-reactivity or interference between CES2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CES2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CES2 ELISA Kit allows for the in vitro quantitative determination of CES2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: CES2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CES2 antibody and an CES2-HRP conjugate. The assay sample and buffer are incubated together with CES2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CES2 concentration since CES2 from samples and CES2-HRP conjugate compete for the anti-CES2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CES2 from the sample, fewer sites are left to bind CES2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CES2 concentration in each sample is interpolated from this standard curve.
Human Cocaine esterase Protein information
Uniprot ID EST2_HUMAN
Uniprot AC O00748; A8K367; Q16859; Q5MAB8; Q7Z366; Q8IUP4; Q8TCP8;
UniGene Hs.282975;
GeneID 8824
KEGG hsa:8824;
Human Cocaine esterase Protein SEQUENCE
SEQUENCE 559 AA; 61807 MW; E2EBCABA2995339A CRC64;
MRLHRLRARL SAVACGLLLL LVRGQGQDSA SPIRTTHTGQ VLGSLVHVKG
ANAGVQTFLG IPFAKPPLGP LRFAPPEPPE SWSGVRDGTT HPAMCLQDLT
AVESEFLSQF NMTFPSDSMS EDCLYLSIYT PAHSHEGSNL PVMVWIHGGA
LVFGMASLYD GSMLAALENV VVVIIQYRLG VLGFFSTGDK HATGNWGYLD
QVAALRWVQQ NIAHFGGNPD RVTIFGESAG GTSVSSLVVS PISQGLFHGA
IMESGVALLP GLIASSADVI STVVANLSAC DQVDSEALVG CLRGKSKEEI
LAINKPFKMI PGVVDGVFLP RHPQELLASA DFQPVPSIVG VNNNEFGWLI
PKVMRIYDTQ KEMDREASQA ALQKMLTLLM LPPTFGDLLR EEYIGDNGDP
QTLQAQFQEM MADSMFVIPA LQVAHFQCSR APVYFYEFQH QPSWLKNIRP
PHMKADHGDE LPFVFRSFFG GNYIKFTEEE EQLSRKMMKY WANFARNGNP
NGEGLPHWPL FDQEEQYLQL NLQPAVGRAL KAHRLQFWKK ALPQKIQELE
EPEERHTEL
UCSC uc002eqq.4; human. [O00748-1];

Category

Human CES2 ELISA Kit