Human SCT ELISA Kit

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  • Alternative name

    Human secretin ELISA Kit;Human prepro-secretin ELISA Kit;

  • Catalog
    E034463
  • species
    Human
  • GeneSCT
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Secretin (SCT). No significant cross-reactivity or interference between Secretin (SCT) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 7.36pg/mL.
  • Intended UseHuman SCT ELISA Kit allows for the in vitro quantitative determination of SCT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Secretin (SCT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Secretin (SCT) and unlabeled Secretin (SCT) (Standards or samples) with the pre-coated antibody specific to Secretin (SCT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Secretin (SCT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Secretin (SCT) in the sample.
  • Human Secretin Protein information
  • Uniprot ID SECR_HUMAN
  • Uniprot AC P09683;
  • UniGene Hs.632324;
  • GeneID 6343
  • KEGG hsa:6343;
  • Human Secretin Protein SEQUENCE
  • SEQUENCE 121 AA; 13016 MW; 44BDB4EFC0E161CF CRC64;

    MAPRPLLLLL LLLGGSAARP APPRARRHSD GTFTSELSRL REGARLQRLL

    QGLVGKRSEQ DAENSMAWTR LSAGLLCPSG SNMPILQAWM PLDGTWSPWL

    PPGPMVSEPA GAAAEGTLRP R

  • UCSC uc001lqo.2; human.;



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