Human ADA ELISA Kit

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  • Alternative name

    Human adenosine aminohydrolase ELISA Kit;Human ADA1 ELISA Kit;Human adenosine deaminase ELISA Kit;

  • Catalog
    E000650
  • species
    Human
  • GeneADA
  • Standard CurveHuman ADA ELISA Kit
  • Other Species Mouse Ada ELISA KitBovine ADA ELISA KitRat Ada ELISA Kit
  • SpecificityNatural and recombinant human ADA
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Sample For quantitative detection of human ADA in cell culture supernates, serum, tissue ho
  • Intended UseHuman ADA ELISA Kit allows for the in vitro quantitative determination of ADA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: Human ADA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADA has been precoated onto 96-well plates. Standards(Expression system for standard: sf21, Immunogen sequence: A2-L363) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ADA amount of sample captured in plate. Background: Adenosine Deaminase (also known as adenosine aminohydrolase, or ADA) is an enzyme involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues. ADA in humans is involved in the development and maintenance of the immune system. However, ADA association has also been observed with epithelial cell differentiation, neurotransmission, and gestation maintenance. It has also been proposed that ADA, in addition to adenosine breakdown, stimulates release of excitatory amino acids and is necessary to the coupling of A1 adenosine receptors and heterotrimeric G proteins. Adenosine deaminase deficiency leads to pulmonary fibrosis, suggesting that chronic exposure to high levels of adenosine can exacerbate inflammation responses rather than suppressing them. It has also been recognized that adenosine deaminase protein and activity is upregulated in mouse hearts that overexpress HIF-1 alpha, which in part explains the attenuated levels of adenosine in HIF-1 alpha expressing hearts during ischemic stress.
  • Human Adenosine deaminase Protein information
  • Uniprot ID ADA_HUMAN
  • Uniprot AC P00813; Q53F92; Q6LA59;
  • UniGene Hs.654536;
  • GeneID 100
  • KEGG hsa:100;
  • Human Adenosine deaminase Protein SEQUENCE
  • SEQUENCE 363 AA; 40764 MW; 786BC5085CA9AFCB CRC64;

    MAQTPAFDKP KVELHVHLDG SIKPETILYY GRRRGIALPA NTAEGLLNVI

    GMDKPLTLPD FLAKFDYYMP AIAGCREAIK RIAYEFVEMK AKEGVVYVEV

    RYSPHLLANS KVEPIPWNQA EGDLTPDEVV ALVGQGLQEG ERDFGVKARS

    ILCCMRHQPN WSPKVVELCK KYQQQTVVAI DLAGDETIPG SSLLPGHVQA

    YQEAVKSGIH RTVHAGEVGS AEVVKEAVDI LKTERLGHGY HTLEDQALYN

    RLRQENMHFE ICPWSSYLTG AWKPDTEHAV IRLKNDQANY SLNTDDPLIF

    KSTLDTDYQM TKRDMGFTEE EFKRLNINAA KSSFLPEDEK RELLDLLYKA

    YGMPPSASAG QNL

  • UCSC uc002xmj.4; human.;


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