Human SPP2 ELISA Kit

Alternative name
Human SPP-24 ELISA Kit;Human secreted phosphoprotein 2 ELISA Kit;Human SPP24 ELISA Kit;Human secreted phosphoprotein 24 ELISA Kit;Human secreted phosphoprotein 2, 24kD ELISA Kit;Human secreted phosphoprotein 2, 24kDa ELISA Kit;
Catalog
E034450
species
Human
GeneSPP2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Secreted Phosphoprotein 2 (SPP2). No significant cross-reactivity or interference between Secreted Phosphoprotein 2 (SPP2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 26.7pg/mL.
Intended UseHuman SPP2 ELISA Kit allows for the in vitro quantitative determination of SPP2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secreted Phosphoprotein 2 (SPP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secreted Phosphoprotein 2 (SPP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secreted Phosphoprotein 2 (SPP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secreted Phosphoprotein 2 (SPP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Secreted phosphoprotein 24 Protein information
Uniprot ID SPP24_HUMAN
Uniprot AC Q13103; A4QMV3; Q3B892; Q546M5;
UniGene Hs.444488;
GeneID 6694
KEGG hsa:6694;
Human Secreted phosphoprotein 24 Protein SEQUENCE
SEQUENCE 211 AA; 24338 MW; AED049E63464E38D CRC64;
MISRMEKMTM MMKILIMFAL GMNYWSCSGF PVYDYDPSSL RDALSASVVK
VNSQSLSPYL FRAFRSSLKR VEVLDENNLV MNLEFSIRET TCRKDSGEDP
ATCAFQRDYY VSTAVCRSTV KVSAQQVQGV HARCSWSSST SESYSSEEMI
FGDMLGSHKW RNNYLFGLIS DESISEQFYD RSLGIMRRVL PPGNRRYPNH
RHRARINTDF E
UCSC uc002vvk.2; human.;

Category

Human SPP2 ELISA Kit

Human SPP-24 ELISA Kit;Human secreted phosphoprotein 2 ELISA Kit;Human SPP24 ELISA Kit;Human secreted phosphoprotein 24 ELISA Kit;Human secreted phosphoprotein 2, 24kD ELISA Kit;Human secreted phosphoprotein 2, 24kDa ELISA Kit;