Human DIAPH1 ELISA Kit

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  • Alternative name

    Human Diaphanous-related formin-1 ELISA Kit;Human DRF1 ELISA Kit;Human DIAP1 ELISA Kit;Human DFNA1 ELISA Kit;Human DIA1 ELISA Kit;Human LFHL1 ELISA Kit;Human SCBMS ELISA Kit;Human hDIA1 ELISA Kit;Human diaphanous related formin 1 ELISA Kit;Human protein diaphanous homolog 1 ELISA Kit;

  • Catalog
    E011874
  • species
    Human
  • GeneDIAPH1
  • Standard CurveHuman DIAPH1 ELISA Kit
  • Other Species Mouse Diaph1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Diaphanous Homolog 1 (DIAPH1). No significant cross-reactivity or interference between Diaphanous Homolog 1 (DIAPH1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.051ng/mL.
  • Intended UseHuman DIAPH1 ELISA Kit allows for the in vitro quantitative determination of DIAPH1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Diaphanous Homolog 1 (DIAPH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Diaphanous Homolog 1 (DIAPH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Diaphanous Homolog 1 (DIAPH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Diaphanous Homolog 1 (DIAPH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Protein diaphanous homolog 1 Protein information
  • Uniprot ID DIAP1_HUMAN
  • Uniprot AC O60610; A6NF18; B7ZKW2; E9PEZ2; Q17RN4; Q59FH8; Q9UC76;
  • UniGene Hs.529451;
  • GeneID 1729
  • KEGG hsa:1729;
  • Human Protein diaphanous homolog 1 Protein SEQUENCE
  • SEQUENCE 1272 AA; 141347 MW; 9C8273DE4748564F CRC64;

    MEPPGGSLGP GRGTRDKKKG RSPDELPSAG GDGGKSKKFT LKRLMADELE

    RFTSMRIKKE KEKPNSAHRN SSASYGDDPT AQSLQDVSDE QVLVLFEQML

    LDMNLNEEKQ QPLREKDIII KREMVSQYLY TSKAGMSQKE SSKSAMMYIQ

    ELRSGLRDMP LLSCLESLRV SLNNNPVSWV QTFGAEGLAS LLDILKRLHD

    EKEETAGSYD SRNKHEIIRC LKAFMNNKFG IKTMLETEEG ILLLVRAMDP

    AVPNMMIDAA KLLSALCILP QPEDMNERVL EAMTERAEMD EVERFQPLLD

    GLKSGTTIAL KVGCLQLINA LITPAEELDF RVHIRSELMR LGLHQVLQDL

    REIENEDMRV QLNVFDEQGE EDSYDLKGRL DDIRMEMDDF NEVFQILLNT

    VKDSKAEPHF LSILQHLLLV RNDYEARPQY YKLIEECISQ IVLHKNGADP

    DFKCRHLQIE IEGLIDQMID KTKVEKSEAK AAELEKKLDS ELTARHELQV

    EMKKMESDFE QKLQDLQGEK DALHSEKQQI ATEKQDLEAE VSQLTGEVAK

    LTKELEDAKK EMASLSAAAI TVPPSVPSRA PVPPAPPLPG DSGTIIPPPP

    APGDSTTPPP PPPPPPPPPP LPGGVCISSP PSLPGGTAIS PPPPLSGDAT

    IPPPPPLPEG VGIPSPSSLP GGTAIPPPPP LPGSARIPPP PPPLPGSAGI

    PPPPPPLPGE AGMPPPPPPL PGGPGIPPPP PFPGGPGIPP PPPGMGMPPP

    PPFGFGVPAA PVLPFGLTPK KLYKPEVQLR RPNWSKLVAE DLSQDCFWTK

    VKEDRFENNE LFAKLTLTFS AQTKTSKAKK DQEGGEEKKS VQKKKVKELK

    VLDSKTAQNL SIFLGSFRMP YQEIKNVILE VNEAVLTESM IQNLIKQMPE

    PEQLKMLSEL KDEYDDLAES EQFGVVMGTV PRLRPRLNAI LFKLQFSEQV

    ENIKPEIVSV TAACEELRKS ESFSNLLEIT LLVGNYMNAG SRNAGAFGFN

    ISFLCKLRDT KSTDQKMTLL HFLAELCEND YPDVLKFPDE LAHVEKASRV

    SAENLQKNLD QMKKQISDVE RDVQNFPAAT DEKDKFVEKM TSFVKDAQEQ

    YNKLRMMHSN METLYKELGE YFLFDPKKLS VEEFFMDLHN FRNMFLQAVK

    ENQKRRETEE KMRRAKLAKE KAEKERLEKQ QKREQLIDMN AEGDETGVMD

    SLLEALQSGA AFRRKRGPRQ ANRKAGCAVT SLLASELTKD DAMAAVPAKV

    SKNSETFPTI LEEAKELVGR AS

  • UCSC uc063iba.1; human. [O60610-1];


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