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Alternative name
Human SAA ELISA Kit;Human Amyloid fibril protein AA ELISA Kit;Human PIG4 ELISA Kit;Human SAA2 ELISA Kit;Human TP53I4 ELISA Kit;Human serum amyloid A1 ELISA Kit;Human serum amyloid A-1 protein ELISA Kit;Human serum amyloid A protein ELISA Kit;Human tumor protein p53 inducible protein 4 ELISA Kit;
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Catalog
E034158
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species
Human
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GeneSAA1
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SpecificityNatural and recombinant Human SAA/SAA1
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity Intra-assay Precision Intra-Assay Precision (Precision within an assay) Three samples of kn
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Intended UseHuman SAA1 ELISA Kit allows for the in vitro quantitative determination of SAA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the Assay: Human SAA/SAA1 ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for SAA/SAA1 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: S20-Y122) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SAA/SAA1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human SAA/SAA1 amount of sample captured in plate.
Background/Introduction: Serum amyloid A protein (SAA), also known as SAA1, is a protein that in humans is encoded by the SAA1 gene. This gene encodes a member of the serum amyloid A family of apolipoproteins. It is mapped to 11p15.1. SAA is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease.This protein may also be a potential biomarker for certain tumors.
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Human Serum amyloid protein A(4-101) Protein information
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Uniprot ID
SAA1_HUMAN
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Uniprot AC
Q3KRB3; Q6FG67; Q96QN0; Q9UCK9; Q9UCL0;
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UniGene
Hs.632144; Hs.731376; Hs.734161;
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GeneID
6288
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KEGG
hsa:6288;
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Human Serum amyloid protein A(4-101) Protein SEQUENCE
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SEQUENCE 122 AA; 13532 MW; 43A57D56B37CB173 CRC64;
MKLLTGLVFC SLVLGVSSRS FFSFLGEAFD GARDMWRAYS DMREANYIGS
DKYFHARGNY
DAAKRGPGGV WAAEAISDAR ENIQRFFGHG AEDSLADQAA
NEWGRSGKDP NHFRPAGLPE
KY
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UCSC
uc057zpu.1; human.;
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