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Alternative name
Coagulation factor XIII B chain ELISA KIT; Fibrin-stabilizing factor B subunit ELISA KIT; Protein-glutamine gamma-glutamyltransferase B chain ELISA KIT; Transglutaminase B chainF13B ELISA KIT;
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Catalog
E048825
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species
Mouse
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GeneF XIII B
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity<1.875ng/ml
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Intended UseMouse F XIII B ELISA Kit allows for the in vitro quantitative determination of F XIII B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse F XIII B antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse F XIII B antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse F XIII B amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse F XIII B can be calculated.
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