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Human S100A13 ELISA Kit

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  • Alternative name

    Human S100 calcium-binding protein A13 ELISA Kit;Human S100 calcium binding protein A13 ELISA Kit;Human protein S100-A13 ELISA Kit;
  • Catalog
    E034079
  • species
    Human
  • GeneS100A13
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman S100A13 ELISA Kit allows for the in vitro quantitative determination of S100A13 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Principle of the Assay: S100A13 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for S100A13. Standards or samples are then added to the microtiter plate wells and S100A13 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of S100A13 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for S100A13 are added to each well to "sandwich" the S100A13 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain S100A13 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100A13 concentration in each sample is interpolated from this standard curve.
  • Human Protein S100-A13 Protein information
  • Uniprot ID S10AD_HUMAN
  • Uniprot AC Q99584; Q52PI9; Q6FGF8;
  • UniGene Hs.516505;
  • GeneID 6284
  • KEGG hsa:6284;
  • Human Protein S100-A13 Protein SEQUENCE

  • SEQUENCE 98 AA; 11471 MW; 1D1BE57F3CD49E81 CRC64;

    MAAEPLTELE ESIETVVTTF FTFARQEGRK DSLSVNEFKE LVTQQLPHLL

    KDVGSLDEKM KSLDVNQDSE LKFNEYWRLI GELAKEIRKK KDLKIRKK

  • UCSC uc001fcf.5; human.;



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