Human ACP5 ELISA Kit

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  • Alternative name

    Human TR-AP ELISA Kit;Human tartrate-resistant acid ATPase ELISA Kit;Human TrATPase ELISA Kit;Human Type 5 acid phosphatase ELISA Kit;Human HPAP ELISA Kit;Human TRACP5a ELISA Kit;Human TRACP5b ELISA Kit;Human TRAP ELISA Kit;Human acid phosphatase 5, tartrate resistant ELISA Kit;Human tartrate-resistant acid phosphatase type 5 ELISA Kit;Human human purple acid phosphatase ELISA Kit;Human tartrate-resistant acid phosphatase 5a ELISA Kit;Human tartrate-resistant acid phosphatase 5b ELISA Kit;

  • Catalog
    E000300
  • species
    Human
  • GeneACP5
  • Standard CurveHuman ACP5 ELISA Kit
  • Other Species Mouse Acp5 ELISA KitPorcine ACP5 ELISA KitRabbit ACP5 ELISA KitRat Acp5 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of TRACP5. No significant cross-reactivity or interference between TRACP5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TRACP5 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ACP5 ELISA Kit allows for the in vitro quantitative determination of ACP5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This TRACP5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TRACP5. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||TRACP5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TRACP5 antibody and an TRACP5-HRP conjugate. The assay sample and buffer are incubated together with TRACP5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TRACP5 concentration since TRACP5 from samples and TRACP5-HRP conjugate compete for the anti-TRACP5 antibody binding site. Since the number of sites is limited, as more sites are occupied by TRACP5 from the sample, fewer sites are left to bind TRACP5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TRACP5 concentration in each sample is interpolated from this standard curve.
  • Human Tartrate-resistant acid phosphatase type 5 Protein information
  • Uniprot ID PPA5_HUMAN
  • Uniprot AC P13686; A8K3V2; Q2TAB1; Q6IAS6; Q9UCJ5; Q9UCJ6; Q9UCJ7;
  • UniGene Hs.1211;
  • GeneID 54
  • KEGG hsa:54;
  • Human Tartrate-resistant acid phosphatase type 5 Protein SEQUENCE
  • SEQUENCE 325 AA; 36599 MW; 079174A71A5BA264 CRC64;

    MDMWTALLIL QALLLPSLAD GATPALRFVA VGDWGGVPNA PFHTAREMAN

    AKEIARTVQI LGADFILSLG DNFYFTGVQD INDKRFQETF EDVFSDRSLR

    KVPWYVLAGN HDHLGNVSAQ IAYSKISKRW NFPSPFYRLH FKIPQTNVSV

    AIFMLDTVTL CGNSDDFLSQ QPERPRDVKL ARTQLSWLKK QLAAAREDYV

    LVAGHYPVWS IAEHGPTHCL VKQLRPLLAT YGVTAYLCGH DHNLQYLQDE

    NGVGYVLSGA GNFMDPSKRH QRKVPNGYLR FHYGTEDSLG GFAYVEISSK

    EMTVTYIEAS GKSLFKTRLP RRARP

  • UCSC uc002msg.5; human.;


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