Human S100 ELISA Kit

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  • Alternative name

    Protein S100-B ELISA KIT; S-100 protein beta chain ELISA KIT; S-100 protein subunit beta ELISA KIT; S100 calcium-binding protein BS100B ELISA KIT;

  • Catalog
    E034066
  • species
    Human
  • GeneS100
  • Other Species Human S100A1/S100 ELISA KitMouse S100 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of S100. No significant cross-reactivity or interference between S100 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between S100 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman S100 ELISA Kit allows for the in vitro quantitative determination of S100 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This S100 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human S100. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||S100 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for S100. Standards or samples are then added to the microtiter plate wells and S100 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of S100 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for S100 are added to each well to "sandwich" the S100 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain S100 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100 concentration in each sample is interpolated from this standard curve.




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