Human RUNX2 ELISA Kit

Alternative name
Human acute myeloid leukemia 3 protein ELISA Kit;Human Core-binding factor subunit alpha-1 ELISA Kit;Human CBF-alpha-1 ELISA Kit;Human oncogene AML-3 ELISA Kit;Human osteoblast-specific transcription factor 2 ELISA Kit;Human OSF-2 ELISA Kit;Human polyomavirus enhancer-binding protein 2 alpha A subunit ELISA Kit;Human PEA2-alpha A ELISA Kit;Human PEBP2-alpha A ELISA Kit;Human SL3-3 enhancer factor 1 alpha A subunit ELISA Kit;Human SL3/AKV core-binding factor alpha A subunit ELISA Kit;Human AML3 ELISA Kit;Human CBFA1 ELISA Kit;Human OSF2 ELISA Kit;Human PEBP2A ELISA Kit;Human CCD ELISA Kit;Human CCD1 ELISA Kit;Human CLCD ELISA Kit;Human PEA2aA ELISA Kit;Human PEBP2aA ELISA Kit;Human runt related transcription factor 2 ELISA Kit;Human runt-related transcription factor 2 ELISA Kit;Human core-binding factor, runt domain, alpha subunit 1 ELISA Kit;
Catalog
E034012
species
Human
GeneRUNX2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Runt Related Transcription Factor 2 (RUNX2). No significant cross-reactivity or interference between Runt Related Transcription Factor 2 (RUNX2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.056ng/mL.
Intended UseHuman RUNX2 ELISA Kit allows for the in vitro quantitative determination of RUNX2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Runt Related Transcription Factor 2 (RUNX2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Runt Related Transcription Factor 2 (RUNX2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Runt Related Transcription Factor 2 (RUNX2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Runt Related Transcription Factor 2 (RUNX2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Runt-related transcription factor 2 Protein information
Uniprot ID RUNX2_HUMAN
Uniprot AC Q13950; O14614; O14615; O95181;
UniGene Hs.535845;
GeneID 860
KEGG hsa:860;
Human Runt-related transcription factor 2 Protein SEQUENCE
SEQUENCE 521 AA; 56648 MW; 44C4F3867D6F3EB1 CRC64;
MASNSLFSTV TPCQQNFFWD PSTSRRFSPP SSSLQPGKMS DVSPVVAAQQ
QQQQQQQQQQ QQQQQQQQQQ QEAAAAAAAA AAAAAAAAAV PRLRPPHDNR
TMVEIIADHP AELVRTDSPN FLCSVLPSHW RCNKTLPVAF KVVALGEVPD
GTVVTVMAGN DENYSAELRN ASAVMKNQVA RFNDLRFVGR SGRGKSFTLT
ITVFTNPPQV ATYHRAIKVT VDGPREPRRH RQKLDDSKPS LFSDRLSDLG
RIPHPSMRVG VPPQNPRPSL NSAPSPFNPQ GQSQITDPRQ AQSSPPWSYD
QSYPSYLSQM TSPSIHSTTP LSSTRGTGLP AITDVPRRIS DDDTATSDFC
LWPSTLSKKS QAGASELGPF SDPRQFPSIS SLTESRFSNP RMHYPATFTY
TPPVTSGMSL GMSATTHYHT YLPPPYPGSS QSQSGPFQTS STPYLYYGTS
SGSYQFPMVP GGDRSPSRML PPCTTTSNGS TLLNPNLPNQ NDGVDADGSH
SSSPTVLNSS GRMDESVWRP Y
UCSC uc003oxt.5; human. [Q13950-1];

Category

Human RUNX2 ELISA Kit