Human ECP ELISA Kit;Human ribonuclease 3 ELISA Kit;Human RNase 3 ELISA Kit;Human RNS3 ELISA Kit;Human RAF1 ELISA Kit;Human ribonuclease A family member 3 ELISA Kit;Human eosinophil cationic protein ELISA Kit;Human cytotoxic ribonuclease ELISA Kit;Human ribonuclease, RNase A family, 3 ELISA Kit;
Catalog
E033600
species
Human
GeneRNASE3
SpecificityThis assay has high sensitivity and excellent specificity for detection of Ribonuclease A3 (RNASE3).
No significant cross-reactivity or interference between Ribonuclease A3 (RNASE3) and analogues was observed.
Intended UseHuman RNASE3 ELISA Kit allows for the in vitro quantitative determination of RNASE3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease A3 (RNASE3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease A3 (RNASE3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease A3 (RNASE3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ribonuclease A3 (RNASE3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Eosinophil cationic protein Protein information
Uniprot ID
ECP_HUMAN
Uniprot AC
P12724; Q4VBC1; Q8WTP7; Q8WZ62; Q9GZN9;
UniGene
Hs.73839;
GeneID
6037
KEGG
hsa:6037;
Human Eosinophil cationic protein Protein SEQUENCE