Human RHD ELISA Kit

Alternative name
Human RHXIII ELISA Kit;Human RH polypeptide 2 ELISA Kit;Human RHPII ELISA Kit;Human rhesus D antigen ELISA Kit;Human CD240D ELISA Kit;Human DIIIc ELISA Kit;Human RH ELISA Kit;Human RH30 ELISA Kit;Human RHCED ELISA Kit;Human RHDVA(TT) ELISA Kit;Human RHDel ELISA Kit;Human Rh4 ELISA Kit;Human RhDCw ELISA Kit;Human RhII ELISA Kit;Human RhK562-II ELISA Kit;Human RhPI ELISA Kit;Human Rh blood group D antigen ELISA Kit;Human blood group Rh(D) polypeptide ELISA Kit;Human D antigen (DCS) ELISA Kit;Human Rh blood group C antigen ELISA Kit;Human Rh blood group CE antigen ELISA Kit;Human Rh blood group CcEe antigen ELISA Kit;Human Rh blood group antigen Evans ELISA Kit;Human Rh blood group, D anitgen ELISA Kit;Human RhD blood group antigen ELISA Kit;Human RhD polypeptide ELISA Kit;Human Rhesus blood group D antigen allele DIII type 7 ELISA Kit;Human Rhesus system D polypeptide ELISA Kit;Human blood group antigen D ELISA Kit;Human blood group protein RHD ELISA Kit;Human truncated Rh blood group D antigen ELISA Kit;Human truncated RhD ELISA Kit;Human truncated rhesus D ELISA Kit;Human truncated rhesus blood group D antigen ELISA Kit;
Catalog
E033454
species
Human
GeneRHD
Standard Curve
Other Species Mouse Rhd ELISA Kit Rat Rhd ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Rh Blood Group, D Antigen (RHD). No significant cross-reactivity or interference between Rh Blood Group, D Antigen (RHD) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.058ng/mL.
Intended UseHuman RHD ELISA Kit allows for the in vitro quantitative determination of RHD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rh Blood Group, D Antigen (RHD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Rh Blood Group, D Antigen (RHD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rh Blood Group, D Antigen (RHD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rh Blood Group, D Antigen (RHD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Blood group Rh(D) polypeptide Protein information
Uniprot ID RHD_HUMAN
Uniprot AC Q5XLT1; Q5XLT2; Q9NPK0; Q9UQ20; Q9UQ21; Q9UQ22; Q9UQ23;
UniGene Hs.449968;
GeneID 6007
KEGG hsa:6007;
Human Blood group Rh(D) polypeptide Protein SEQUENCE
SEQUENCE 417 AA; 45211 MW; 38721BFA664AE199 CRC64;
MSSKYPRSVR RCLPLWALTL EAALILLFYF FTHYDASLED QKGLVASYQV
GQDLTVMAAI GLGFLTSSFR RHSWSSVAFN LFMLALGVQW AILLDGFLSQ
FPSGKVVITL FSIRLATMSA LSVLISVDAV LGKVNLAQLV VMVLVEVTAL
GNLRMVISNI FNTDYHMNMM HIYVFAAYFG LSVAWCLPKP LPEGTEDKDQ
TATIPSLSAM LGALFLWMFW PSFNSALLRS PIERKNAVFN TYYAVAVSVV
TAISGSSLAH PQGKISKTYV HSAVLAGGVA VGTSCHLIPS PWLAMVLGLV
AGLISVGGAK YLPGCCNRVL GIPHSSIMGY NFSLLGLLGE IIYIVLLVLD
TVGAGNGMIG FQVLLSIGEL SLAIVIALMS GLLTGLLLNL KIWKAPHEAK
YFDDQVFWKF PHLAVGF
UCSC uc001bjz.5; human. [Q02161-1];

Category

Human RHD ELISA Kit