Human ALDH1A1 ELISA Kit

Alternative name
Human RALDH 1 ELISA Kit;Human RalDH1 ELISA Kit;Human ALDH-E1 ELISA Kit;Human ALHDII ELISA Kit;Human Aldehyde dehydrogenase family 1 member A1 ELISA Kit;Human Aldehyde dehydrogenase, cytosolic ELISA Kit;Human ALDC ELISA Kit;Human ALDH1 ELISA Kit;Human ALDH11 ELISA Kit;Human HEL-9 ELISA Kit;Human HEL-S-53e ELISA Kit;Human HEL12 ELISA Kit;Human PUMB1 ELISA Kit;Human RALDH1 ELISA Kit;Human aldehyde dehydrogenase 1 family member A1 ELISA Kit;Human retinal dehydrogenase 1 ELISA Kit;Human ALDH class 1 ELISA Kit;Human RALDH 1 ELISA Kit;Human acetaldehyde dehydrogenase 1 ELISA Kit;Human aldehyde dehydrogenase 1, soluble ELISA Kit;Human aldehyde dehydrogenase, liver cytosolic ELISA Kit;Human epididymis luminal protein 12 ELISA Kit;Human epididymis luminal protein 9 ELISA Kit;Human epididymis secretory sperm binding protein Li 53e ELISA Kit;Human retinaldehyde dehydrogenase 1 ELISA Kit;
Catalog
E033312
species
Human
GeneALDH1A1
SpecificityThis assay has high sensitivity and excellent specificity for detection of ALDH1A1. No significant cross-reactivity or interference between ALDH1A1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ALDH1A1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman ALDH1A1 ELISA Kit allows for the in vitro quantitative determination of ALDH1A1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeuroscience
Product Description
specificalIntended Uses: This ALDH1A1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ALDH1A1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: ALDH1A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ALDH1A1 antibody and an ALDH1A1-HRP conjugate. The assay sample and buffer are incubated together with ALDH1A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALDH1A1 concentration since ALDH1A1 from samples and ALDH1A1-HRP conjugate compete for the anti-ALDH1A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ALDH1A1 from the sample, fewer sites are left to bind ALDH1A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALDH1A1 concentration in each sample is interpolated from this standard curve.
Human Retinal dehydrogenase 1 Protein information
Uniprot ID AL1A1_HUMAN
Uniprot AC P00352; O00768; Q5SYR1;
UniGene Hs.76392;
GeneID 216
KEGG hsa:216;
Human Retinal dehydrogenase 1 Protein SEQUENCE
SEQUENCE 501 AA; 54862 MW; B26464DC7168348E CRC64;
MSSSGTPDLP VLLTDLKIQY TKIFINNEWH DSVSGKKFPV FNPATEEELC
QVEEGDKEDV DKAVKAARQA FQIGSPWRTM DASERGRLLY KLADLIERDR
LLLATMESMN GGKLYSNAYL NDLAGCIKTL RYCAGWADKI QGRTIPIDGN
FFTYTRHEPI GVCGQIIPWN FPLVMLIWKI GPALSCGNTV VVKPAEQTPL
TALHVASLIK EAGFPPGVVN IVPGYGPTAG AAISSHMDID KVAFTGSTEV
GKLIKEAAGK SNLKRVTLEL GGKSPCIVLA DADLDNAVEF AHHGVFYHQG
QCCIAASRIF VEESIYDEFV RRSVERAKKY ILGNPLTPGV TQGPQIDKEQ
YDKILDLIES GKKEGAKLEC GGGPWGNKGY FVQPTVFSNV TDEMRIAKEE
IFGPVQQIMK FKSLDDVIKR ANNTFYGLSA GVFTKDIDKA ITISSALQAG
TVWVNCYGVV SAQCPFGGFK MSGNGRELGE YGFHEYTEVK TVTVKISQKN
S

Category

Human ALDH1A1 ELISA Kit