Mouse Il21 ELISA Kit

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  • Alternative name

    Mouse IL-21 ELISA Kit;Mouse interleukin 21 ELISA Kit;Mouse interleukin-21 ELISA Kit;

  • Catalog
    E056342
  • species
    Mouse
  • GeneIl21
  • Other Species Human IL21 ELISA KitBovine IL21 ELISA KitCanine IL21 ELISA KitPorcine IL21 ELISA KitRat Il21 ELISA Kit
  • SpecificityNatural and recombinant mouse IL-21
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10pg/ml
  • Intended UseMouse Il21 ELISA Kit allows for the in vitro quantitative determination of Il21 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: mouse IL-21 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-21 has been precoated onto 96-well plates. Standards(E.coli,P25-S146) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-21 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-21 amount of sample captured in plate. Background: Interleukin-21 is a protein that in humans is encoded by the IL21 gene. Interleukin 21 is a cytokine that has potent regulatory effects on cells of the immune system, including natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells. By radiation hybrid analysis, the IL21 gene is mapped to chromosome 4q26-q27, near the IL2 gene. IL21 stimulates B-cell proliferation in an anti-CD40-dependent manner but inhibits proliferation stimulated by IL4 plus anti-IgM. The biologic effects of IL21 on NK cells differ between species, with human IL21 enhancing NK cell proliferation and murine IL21 inhibiting NK cell proliferation. IL21 is a cytokine highly expressed by mouse T(H)17 cells.
  • Mouse Interleukin-21 Protein information
  • Uniprot ID IL21_MOUSE
  • Uniprot AC Q9ES17;
  • UniGene Mm.157689; Mm.393490;
  • GeneID 60505
  • KEGG mmu:60505;
  • Mouse Interleukin-21 Protein SEQUENCE
  • SEQUENCE 146 AA; 16811 MW; D2527ED95BA15194 CRC64;

    MERTLVCLVV IFLGTVAHKS SPQGPDRLLI RLRHLIDIVE QLKIYENDLD

    PELLSAPQDV KGHCEHAAFA CFQKAKLKPS NPGNNKTFII DLVAQLRRRL

    PARRGGKKQK HIAKCPSCDS YEKRTPKEFL ERLKWLLQKM IHQHLS

  • UCSC uc008paj.2; mouse.;



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