Mouse Il21 ELISA Kit

Alternative name
Mouse IL-21 ELISA Kit;Mouse interleukin 21 ELISA Kit;Mouse interleukin-21 ELISA Kit;
Catalog
E056342
species
Mouse
GeneIl21
SpecificityNatural and recombinant mouse IL-21
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseMouse Il21 ELISA Kit allows for the in vitro quantitative determination of Il21 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: mouse IL-21 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-21 has been precoated onto 96-well plates. Standards(E.coli,P25-S146) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-21 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-21 amount of sample captured in plate. Background: Interleukin-21 is a protein that in humans is encoded by the IL21 gene. Interleukin 21 is a cytokine that has potent regulatory effects on cells of the immune system, including natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells. By radiation hybrid analysis, the IL21 gene is mapped to chromosome 4q26-q27, near the IL2 gene. IL21 stimulates B-cell proliferation in an anti-CD40-dependent manner but inhibits proliferation stimulated by IL4 plus anti-IgM. The biologic effects of IL21 on NK cells differ between species, with human IL21 enhancing NK cell proliferation and murine IL21 inhibiting NK cell proliferation. IL21 is a cytokine highly expressed by mouse T(H)17 cells.
Mouse Interleukin-21 Protein information
Uniprot ID IL21_MOUSE
Uniprot AC Q9ES17;
UniGene Mm.157689; Mm.393490;
GeneID 60505
KEGG mmu:60505;
Mouse Interleukin-21 Protein SEQUENCE
SEQUENCE 146 AA; 16811 MW; D2527ED95BA15194 CRC64;
MERTLVCLVV IFLGTVAHKS SPQGPDRLLI RLRHLIDIVE QLKIYENDLD
PELLSAPQDV KGHCEHAAFA CFQKAKLKPS NPGNNKTFII DLVAQLRRRL
PARRGGKKQK HIAKCPSCDS YEKRTPKEFL ERLKWLLQKM IHQHLS
UCSC uc008paj.2; mouse.;

Mouse Il21 ELISA Kit

Mouse IL-21 ELISA Kit;Mouse interleukin 21 ELISA Kit;Mouse interleukin-21 ELISA Kit;

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