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Human RCN1 ELISA Kit

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  • Alternative name

    Human RCN ELISA Kit;Human HEL-S-84 ELISA Kit;Human PIG20 ELISA Kit;Human RCAL ELISA Kit;Human reticulocalbin 1 ELISA Kit;Human reticulocalbin-1 ELISA Kit;Human epididymis secretory protein Li 84 ELISA Kit;Human proliferation-inducing gene 20 ELISA Kit;Human reticulocalbin 1, EF-hand calcium binding domain ELISA Kit;
  • Catalog
    E033284
  • species
    Human
  • GeneRCN1
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.375ng/ml
  • Intended UseHuman RCN1 ELISA Kit allows for the in vitro quantitative determination of RCN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human RCN1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human RCN1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human RCN1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human RCN1 can be calculated.
  • Human Reticulocalbin-1 Protein information
  • Uniprot ID RCN1_HUMAN
  • Uniprot AC Q15293; B7Z1M1; D3DR00;
  • UniGene Hs.97887;
  • GeneID 5954
  • KEGG hsa:5954;
  • Human Reticulocalbin-1 Protein SEQUENCE

  • SEQUENCE 331 AA; 38890 MW; 608AAD536963F789 CRC64;

    MARGGRGRRL GLALGLLLAL VLAPRVLRAK PTVRKERVVR PDSELGERPP

    EDNQSFQYDH EAFLGKEDSK TFDQLTPDES KERLGKIVDR IDNDGDGFVT

    TEELKTWIKR VQKRYIFDNV AKVWKDYDRD KDDKISWEEY KQATYGYYLG

    NPAEFHDSSD HHTFKKMLPR DERRFKAADL NGDLTATREE FTAFLHPEEF

    EHMKEIVVLE TLEDIDKNGD GFVDQDEYIA DMFSHEENGP EPDWVLSERE

    QFNEFRDLNK DGKLDKDEIR HWILPQDYDH AQAEARHLVY ESDKNKDEKL

    TKEEILENWN MFVGSQATNY GEDLTKNHDE L

  • UCSC uc058acp.1; human. [Q15293-1];



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