Human HIF2a ELISA Kit

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  • Alternative name

    Endothelial PAS domain-containing protein 1 ELISA KIT; Hypoxia-inducible factor 2-alpha ELISA KIT; HIF-2-alpha ELISA KIT; HIF2-alphaEpas1 ELISA KIT; Hif2a ELISA KIT; EPAS-1 ELISA KIT; HIF-2-alpha ELISA KIT; HIF2-alpha ELISA KIT

  • Catalog
    E019186
  • species
    Human
  • GeneHIF2a
  • Standard CurveHuman HIF2a ELISA Kit
  • Other Species Mouse HIF2a ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Hypoxia Inducible Factor 2 Alpha (HIF2a). No significant cross-reactivity or interference between Hypoxia Inducible Factor 2 Alpha (HIF2a) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.057ng/mL.
  • Intended UseHuman HIF2a ELISA Kit allows for the in vitro quantitative determination of HIF2a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hypoxia Inducible Factor 2 Alpha (HIF2a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hypoxia Inducible Factor 2 Alpha (HIF2a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hypoxia Inducible Factor 2 Alpha (HIF2a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hypoxia Inducible Factor 2 Alpha (HIF2a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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