Human VHL ELISA Kit

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  • Alternative name

    Human protein G7 ELISA Kit;Human pVHL ELISA Kit;Human HRCA1 ELISA Kit;Human RCA1 ELISA Kit;Human VHL1 ELISA Kit;Human von Hippel-Lindau tumor suppressor ELISA Kit;Human von Hippel-Lindau disease tumor suppressor ELISA Kit;Human elongin binding protein ELISA Kit;Human von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase ELISA Kit;

  • Catalog
    E040420
  • species
    Human
  • GeneVHL
  • Other Species Mouse Vhl ELISA KitCanine VHL ELISA KitRat Vhl ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.094ng/ml
  • Intended UseHuman VHL ELISA Kit allows for the in vitro quantitative determination of VHL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human VHL antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human VHL antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human VHL amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human VHL can be calculated.
  • Human Von Hippel-Lindau disease tumor suppressor Protein information
  • Uniprot ID VHL_HUMAN
  • Uniprot AC P40337; B2RE45; Q13599; Q6PDA9;
  • UniGene Hs.517792;
  • GeneID 7428
  • KEGG hsa:7428;
  • Human Von Hippel-Lindau disease tumor suppressor Protein SEQUENCE
  • SEQUENCE 213 AA; 24153 MW; BA5D6765FBC16EA7 CRC64;

    MPRRAENWDE AEVGAEEAGV EEYGPEEDGG EESGAEESGP EESGPEELGA

    EEEMEAGRPR PVLRSVNSRE PSQVIFCNRS PRVVLPVWLN FDGEPQPYPT

    LPPGTGRRIH SYRGHLWLFR DAGTHDGLLV NQTELFVPSL NVDGQPIFAN

    ITLPVYTLKE RCLQVVRSLV KPENYRRLDI VRSLYEDLED HPNVQKDLER

    LTQERIAHQR MGD

  • UCSC uc003bvc.4; human. [P40337-1];


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