Human VHL ELISA Kit

Alternative name
Human protein G7 ELISA Kit;Human pVHL ELISA Kit;Human HRCA1 ELISA Kit;Human RCA1 ELISA Kit;Human VHL1 ELISA Kit;Human von Hippel-Lindau tumor suppressor ELISA Kit;Human von Hippel-Lindau disease tumor suppressor ELISA Kit;Human elongin binding protein ELISA Kit;Human von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase ELISA Kit;
Catalog
E040420
species
Human
GeneVHL
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseHuman VHL ELISA Kit allows for the in vitro quantitative determination of VHL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human VHL antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human VHL antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human VHL amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human VHL can be calculated.
Human Von Hippel-Lindau disease tumor suppressor Protein information
Uniprot ID VHL_HUMAN
Uniprot AC P40337; B2RE45; Q13599; Q6PDA9;
UniGene Hs.517792;
GeneID 7428
KEGG hsa:7428;
Human Von Hippel-Lindau disease tumor suppressor Protein SEQUENCE
SEQUENCE 213 AA; 24153 MW; BA5D6765FBC16EA7 CRC64;
MPRRAENWDE AEVGAEEAGV EEYGPEEDGG EESGAEESGP EESGPEELGA
EEEMEAGRPR PVLRSVNSRE PSQVIFCNRS PRVVLPVWLN FDGEPQPYPT
LPPGTGRRIH SYRGHLWLFR DAGTHDGLLV NQTELFVPSL NVDGQPIFAN
ITLPVYTLKE RCLQVVRSLV KPENYRRLDI VRSLYEDLED HPNVQKDLER
LTQERIAHQR MGD
UCSC uc003bvc.4; human. [P40337-1];

Human VHL ELISA Kit

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