Human REPIN1 ELISA Kit

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  • Alternative name

    Human 60 kDa origin-specific DNA-binding protein ELISA Kit;Human 60 kDa replication initiation region protein ELISA Kit;Human ATT-binding protein ELISA Kit;Human DHFR oribeta-binding protein RIP60 ELISA Kit;Human Zinc finger protein 464 ELISA Kit;Human RIP60 ELISA Kit;Human ZNF464 ELISA Kit;Human AP4 ELISA Kit;Human Zfp464 ELISA Kit;Human replication initiator 1 ELISA Kit;Human H_DJ0584D14.12 ELISA Kit;Human replication initiation region protein (60kD) ELISA Kit;Human zinc finger protein 464 (RIP60) ELISA Kit;Human zinc finger protein AP4 ELISA Kit;

  • Catalog
    E033209
  • species
    Human
  • GeneREPIN1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Replication Initiator 1 (REPIN1). No significant cross-reactivity or interference between Replication Initiator 1 (REPIN1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.129ng/mL.
  • Intended UseHuman REPIN1 ELISA Kit allows for the in vitro quantitative determination of REPIN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Replication Initiator 1 (REPIN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Replication Initiator 1 (REPIN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Replication Initiator 1 (REPIN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Replication Initiator 1 (REPIN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Replication initiator 1 Protein information
  • Uniprot ID REPI1_HUMAN
  • Uniprot AC Q9BWE0; C9J3L7; D3DWZ1; Q7LE03; Q9BUZ6; Q9NZH2; Q9UMP5;
  • UniGene Hs.647086; Hs.655734;
  • GeneID 29803
  • KEGG hsa:29803;
  • Human Replication initiator 1 Protein SEQUENCE
  • SEQUENCE 567 AA; 63575 MW; 00D43EC28E176237 CRC64;

    MLERRCRGPL AMGLAQPRLL SGPSQESPQT LGKESRGLRQ QGTSVAQSGA

    QAPGRAHRCA HCRRHFPGWV ALWLHTRRCQ ARLPLPCPEC GRRFRHAPFL

    ALHRQVHAAA TPDLGFACHL CGQSFRGWVA LVLHLRAHSA AKRPIACPKC

    ERRFWRRKQL RAHLRRCHPP APEARPFICG NCGRSFAQWD QLVAHKRVHV

    AEALEEAAAK ALGPRPRGRP AVTAPRPGGD AVDRPFQCAC CGKRFRHKPN

    LIAHRRVHTG ERPHQCPECG KRFTNKPYLT SHRRIHTGEK PYPCKECGRR

    FRHKPNLLSH SKIHKRSEGS AQAAPGPGSP QLPAGPQESA AEPTPAVPLK

    PAQEPPPGAP PEHPQDPIEA PPSLYSCDDC GRSFRLERFL RAHQRQHTGE

    RPFTCAECGK NFGKKTHLVA HSRVHSGERP FACEECGRRF SQGSHLAAHR

    RDHAPDRPFV CPDCGKAFRH KPYLAAHRRI HTGEKPYVCP DCGKAFSQKS

    NLVSHRRIHT GERPYACPDC DRSFSQKSNL ITHRKSHIRD GAFCCAICGQ

    TFDDEERLLA HQKKHDV

  • UCSC uc003whc.2; human. [Q9BWE0-3];



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