Human ZFAND2B ELISA Kit

Alternative name
Human arsenite-inducible RNA-associated protein-like protein ELISA Kit;Human AIRAP-like protein ELISA Kit;Human AIRAPL ELISA Kit;Human zinc finger AN1-type containing 2B ELISA Kit;Human AN1-type zinc finger protein 2B ELISA Kit;Human zinc finger, AN1-type domain 2B ELISA Kit;
Catalog
E001936
species
Human
GeneZFAND2B
Standard Curve
Other Species Mouse Zfand2b ELISA Kit Rat Zfand2b ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of lupus anticoagulant antibody (IgG). No significant cross-reactivity or interference between lupus anticoagulant antibody (IgG) and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between lupus anticoagulant antibody (IgG) and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman ZFAND2B ELISA Kit allows for the in vitro quantitative determination of ZFAND2B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the assay: lupus anticoagulant antibody (IgG) ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-lupus anticoagulant antibody (IgG) antibody and an lupus anticoagulant antibody (IgG) -HRP conjugate. The assay sample and buffer are incubated together with lupus anticoagulant antibody (IgG) -HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the lupus anticoagulant antibody (IgG) concentration since lupus anticoagulant antibody (IgG) from samples and lupus anticoagulant antibody (IgG) -HRP conjugate compete for the anti-lupus anticoagulant antibody (IgG) antibody binding site. Since the number of sites is limited, as more sites are occupied by lupus anticoagulant antibody (IgG) from the sample, fewer sites are left to bind lupus anticoagulant antibody (IgG) -HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The lupus anticoagulant antibody (IgG) concentration in each sample is interpolated from this standard curve.
Human AN1-type zinc finger protein 2B Protein information
Uniprot ID ZFN2B_HUMAN
Uniprot AC Q8WV99; Q8NB98;
UniGene Hs.534540;
GeneID 130617
KEGG hsa:130617;
Human AN1-type zinc finger protein 2B Protein SEQUENCE
SEQUENCE 257 AA; 28023 MW; E87D47744157F48C CRC64;
MEFPDLGAHC SEPSCQRLDF LPLKCDACSG IFCADHVAYA QHHCGSAYQK
DIQVPVCPLC NVPVPVARGE PPDRAVGEHI DRDCRSDPAQ QKRKIFTNKC
ERAGCRQREM MKLTCERCSR NFCIKHRHPL DHDCSGEGHP TSRAGLAAIS
RAQAVASTST VPSPSQTMPS CTSPSRATTR SPSWTAPPVI ALQNGLSEDE
ALQRALEMSL AETKPQVPSC QEEEDLALAQ ALSASEAEYQ RQQAQSRSSK
PSNCSLC
UCSC uc002vjz.3; human. [Q8WV99-1];

Category

Human ZFAND2B ELISA Kit

Human arsenite-inducible RNA-associated protein-like protein ELISA Kit;Human AIRAP-like protein ELISA Kit;Human AIRAPL ELISA Kit;Human zinc finger AN1-type containing 2B ELISA Kit;Human AN1-type zinc finger protein 2B ELISA Kit;Human zinc finger, AN1-type domain 2B ELISA Kit;