Human POMGNT1 ELISA Kit

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  • Alternative name

    Human POMGnT1 ELISA Kit;Human UDP-GlcNAc:alpha-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I.2 ELISA Kit;Human GnT I.2 ELISA Kit;Human MGAT1.2 ELISA Kit;Human GNTI.2 ELISA Kit;Human LGMD2O ELISA Kit;Human MEB ELISA Kit;Human RP76 ELISA Kit;Human gnT-I.2 ELISA Kit;Human protein O-linked mannose N-acetylglucosaminyltransferase 1 (beta 1,2-) ELISA Kit;Human protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 ELISA Kit;

  • Catalog
    E031688
  • species
    Human
  • GenePOMGNT1
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.094ng/ml
  • Intended UseHuman POMGNT1 ELISA Kit allows for the in vitro quantitative determination of POMGNT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-POMGNT1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-POMGNT1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the POMGNT1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of POMGNT1 can be calculated.
  • Human Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 Protein information
  • Uniprot ID PMGT1_HUMAN
  • Uniprot AC Q8WZA1; D3DQ16; Q5VST2; Q5VST3; Q9BV55; Q9H9L8; Q9NXF9; Q9NYF7;
  • UniGene Hs.525134;
  • GeneID 55624
  • KEGG hsa:55624;
  • Human Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 Protein SEQUENCE
  • SEQUENCE 660 AA; 75252 MW; C58D0E543E033F17 CRC64;

    MDDWKPSPLI KPFGARKKRS WYLTWKYKLT NQRALRRFCQ TGAVLFLLVT

    VIVNIKLILD TRRAISEANE DPEPEQDYDE ALGRLEPPRR RGSGPRRVLD

    VEVYSSRSKV YVAVDGTTVL EDEAREQGRG IHVIVLNQAT GHVMAKRVFD

    TYSPHEDEAM VLFLNMVAPG RVLICTVKDE GSFHLKDTAK ALLRSLGSQA

    GPALGWRDTW AFVGRKGGPV FGEKHSKSPA LSSWGDPVLL KTDVPLSSAE

    EAECHWADTE LNRRRRRFCS KVEGYGSVCS CKDPTPIEFS PDPLPDNKVL

    NVPVAVIAGN RPNYLYRMLR SLLSAQGVSP QMITVFIDGY YEEPMDVVAL

    FGLRGIQHTP ISIKNARVSQ HYKASLTATF NLFPEAKFAV VLEEDLDIAV

    DFFSFLSQSI HLLEEDDSLY CISAWNDQGY EHTAEDPALL YRVETMPGLG

    WVLRRSLYKE ELEPKWPTPE KLWDWDMWMR MPEQRRGREC IIPDVSRSYH

    FGIVGLNMNG YFHEAYFKKH KFNTVPGVQL RNVDSLKKEA YEVEVHRLLS

    EAEVLDHSKN PCEDSFLPDT EGHTYVAFIR MEKDDDFTTW TQLAKCLHIW

    DLDVRGNHRG LWRLFRKKNH FLMVGVPASP YSVKKPPSVT PIFLEPPPKE

    EGAPGAPEQT

  • UCSC uc001cpe.4; human. [Q8WZA1-1];



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