Human DKK1 ELISA Kit

Alternative name
Human Dickkopf-1 ELISA Kit;Human DKK-1 ELISA Kit;Human hDkk-1 ELISA Kit;Human SK ELISA Kit;Human dickkopf WNT signaling pathway inhibitor 1 ELISA Kit;Human dickkopf-related protein 1 ELISA Kit;Human dickkopf 1 homolog ELISA Kit;Human dickkopf-1 like ELISA Kit;Human dickkopf-like protein 1 ELISA Kit;
Catalog
E011883
species
Human
GeneDKK1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human DKK1. No significant cross-reactivity or interference between Human DKK1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity15.6pg/ml
Intended UseHuman DKK1 ELISA Kit allows for the in vitro quantitative determination of DKK1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DKK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DKK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DKK1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DKK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Dickkopf-related protein 1 Protein information
Uniprot ID DKK1_HUMAN
Uniprot AC O94907; B2RC19;
UniGene Hs.40499;
GeneID 22943
KEGG hsa:22943;
Human Dickkopf-related protein 1 Protein SEQUENCE
SEQUENCE 266 AA; 28672 MW; 5E878B2CCE4236BA CRC64;
MMALGAAGAT RVFVAMVAAA LGGHPLLGVS ATLNSVLNSN AIKNLPPPLG
GAAGHPGSAV SAAPGILYPG GNKYQTIDNY QPYPCAEDEE CGTDEYCASP
TRGGDAGVQI CLACRKRRKR CMRHAMCCPG NYCKNGICVS SDQNHFRGEI
EETITESFGN DHSTLDGYSR RTTLSSKMYH TKGQEGSVCL RSSDCASGLC
CARHFWSKIC KPVLKEGQVC TKHRRKGSHG LEIFQRCYCG EGLSCRIQKD
HHQASNSSRL HTCQRH
UCSC uc001jjr.4; human.;

Human DKK1 ELISA Kit

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