Human PKN1 ELISA Kit

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  • Alternative name

    Human protease-activated kinase 1 ELISA Kit;Human PAK-1 ELISA Kit;Human protein kinase C-like 1 ELISA Kit;Human protein kinase C-like PKN ELISA Kit;Human protein kinase PKN-alpha ELISA Kit;Human Protein-kinase C-related kinase 1 ELISA Kit;Human Serine-threonine protein kinase N ELISA Kit;Human PAK1 ELISA Kit;Human PKN ELISA Kit;Human PRK1 ELISA Kit;Human PRKCL1 ELISA Kit;Human DBK ELISA Kit;Human PKN-ALPHA ELISA Kit;Human protein kinase N1 ELISA Kit;Human serine/threonine-protein kinase N1 ELISA Kit;Human protein kinase C-related kinase 1 ELISA Kit;Human serine-threonine kinase N ELISA Kit;Human serine/threonine protein kinase N ELISA Kit;

  • Catalog
    E031644
  • species
    Human
  • GenePKN1
  • Standard CurveHuman PKN1 ELISA Kit
  • Other Species Mouse Pkn1 ELISA KitBovine PKN1 ELISA KitRat Pkn1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein Kinase N1 (PKN1). No significant cross-reactivity or interference between Protein Kinase N1 (PKN1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.117ng/mL.
  • Intended UseHuman PKN1 ELISA Kit allows for the in vitro quantitative determination of PKN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase N1 (PKN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase N1 (PKN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase N1 (PKN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase N1 (PKN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Serine/threonine-protein kinase N1 Protein information
  • Uniprot ID PKN1_HUMAN
  • Uniprot AC Q16512; A8K7W5; B2R9R4; B3KVN3; Q15143; Q504U4; Q8IUV5; Q9UD44;
  • UniGene Hs.466044;
  • GeneID 5585
  • KEGG hsa:5585;
  • Human Serine/threonine-protein kinase N1 Protein SEQUENCE
  • SEQUENCE 942 AA; 103932 MW; 61360295EC70BB8E CRC64;

    MASDAVQSEP RSWSLLEQLG LAGADLAAPG VQQQLELERE RLRREIRKEL

    KLKEGAENLR RATTDLGRSL GPVELLLRGS SRRLDLLHQQ LQELHAHVVL

    PDPAATHDGP QSPGAGGPTC SATNLSRVAG LEKQLAIELK VKQGAENMIQ

    TYSNGSTKDR KLLLTAQQML QDSKTKIDII RMQLRRALQA GQLENQAAPD

    DTQGSPDLGA VELRIEELRH HFRVEHAVAE GAKNVLRLLS AAKAPDRKAV

    SEAQEKLTES NQKLGLLREA LERRLGELPA DHPKGRLLRE ELAAASSAAF

    STRLAGPFPA THYSTLCKPA PLTGTLEVRV VGCRDLPETI PWNPTPSMGG

    PGTPDSRPPF LSRPARGLYS RSGSLSGRSS LKAEAENTSE VSTVLKLDNT

    VVGQTSWKPC GPNAWDQSFT LELERARELE LAVFWRDQRG LCALKFLKLE

    DFLDNERHEV QLDMEPQGCL VAEVTFRNPV IERIPRLRRQ KKIFSKQQGK

    AFQRARQMNI DVATWVRLLR RLIPNATGTG TFSPGASPGS EARTTGDISV

    EKLNLGTDSD SSPQKSSRDP PSSPSSLSSP IQESTAPELP SETQETPGPA

    LCSPLRKSPL TLEDFKFLAV LGRGHFGKVL LSEFRPSGEL FAIKALKKGD

    IVARDEVESL MCEKRILAAV TSAGHPFLVN LFGCFQTPEH VCFVMEYSAG

    GDLMLHIHSD VFSEPRAIFY SACVVLGLQF LHEHKIVYRD LKLDNLLLDT

    EGYVKIADFG LCKEGMGYGD RTSTFCGTPE FLAPEVLTDT SYTRAVDWWG

    LGVLLYEMLV GESPFPGDDE EEVFDSIVND EVRYPRFLSA EAIGIMRRLL

    RRNPERRLGS SERDAEDVKK QPFFRTLGWE ALLARRLPPP FVPTLSGRTD

    VSNFDEEFTG EAPTLSPPRD ARPLTAAEQA AFLDFDFVAG GC

  • UCSC uc002myp.4; human. [Q16512-1];



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