Human pERK ELISA Kit

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  • Alternative name

    Eukaryotic translation initiation factor 2-alpha kinase 3 ELISA KIT; PRKR-like endoplasmic reticulum kinase ELISA KIT; Pancreatic eIF2-alpha kinase ELISA KIT; HsPEKEIF2AK3 ELISA KIT; PEK ELISA KIT; PERK ELISA KIT; HsPEK ELISA KIT

  • Catalog
  • species
  • GenepERK
  • Other Species Mouse pERK ELISA Kit
  • SpecificityThis kit recognizes natural and recombinant Human pERK. No significant cross-reactivity or interference between Human pERK and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of Human pERK is 18.75pg/mL
  • Intended UseHuman pERK ELISA Kit allows for the in vitro quantitative determination of pERK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Intended Uses||This ELISA kit applies to the in vitro quantitative determination of Human pERK concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to pERK. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for pERK and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain pERK, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of pERK. You can calculate the concentration of pERK in the samples by comparing the OD of the samples to the standard curve.

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