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Human PDIA4 ELISA Kit

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  • Alternative name

    Human endoplasmic reticulum resident protein 70 ELISA Kit;Human ER protein 70 ELISA Kit;Human ERP70 ELISA Kit;Human endoplasmic reticulum resident protein 72 ELISA Kit;Human ER protein 72 ELISA Kit;Human ERp-72 ELISA Kit;Human ERP72 ELISA Kit;Human protein disulfide isomerase family A member 4 ELISA Kit;Human protein disulfide-isomerase A4 ELISA Kit;Human protein disulfide isomerase related protein (calcium-binding protein, intestinal-related) ELISA Kit;Human protein disulfide isomerase-associated 4 ELISA Kit;Human testicular tissue protein Li 137 ELISA Kit;
  • Catalog
    E031525
  • species
    Human
  • GenePDIA4
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein Disulfide Isomerase A4 (PDIA4). No significant cross-reactivity or interference between Protein Disulfide Isomerase A4 (PDIA4) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.65ng/mL.
  • Intended UseHuman PDIA4 ELISA Kit allows for the in vitro quantitative determination of PDIA4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase A4 (PDIA4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Disulfide Isomerase A4 (PDIA4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Disulfide Isomerase A4 (PDIA4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Disulfide Isomerase A4 (PDIA4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Protein disulfide-isomerase A4 Protein information
  • Uniprot ID PDIA4_HUMAN
  • Uniprot AC P13667; A8K4K6; Q549T6;
  • UniGene Hs.93659;
  • GeneID 9601
  • KEGG hsa:9601;
  • Human Protein disulfide-isomerase A4 Protein SEQUENCE

  • SEQUENCE 645 AA; 72932 MW; 1919C2AE12CD2684 CRC64;

    MRPRKAFLLL LLLGLVQLLA VAGAEGPDED SSNRENAIED EEEEEEEDDD

    EEEDDLEVKE ENGVLVLNDA NFDNFVADKD TVLLEFYAPW CGHCKQFAPE

    YEKIANILKD KDPPIPVAKI DATSASVLAS RFDVSGYPTI KILKKGQAVD

    YEGSRTQEEI VAKVREVSQP DWTPPPEVTL VLTKENFDEV VNDADIILVE

    FYAPWCGHCK KLAPEYEKAA KELSKRSPPI PLAKVDATAE TDLAKRFDVS

    GYPTLKIFRK GRPYDYNGPR EKYGIVDYMI EQSGPPSKEI LTLKQVQEFL

    KDGDDVIIIG VFKGESDPAY QQYQDAANNL REDYKFHHTF STEIAKFLKV

    SQGQLVVMQP EKFQSKYEPR SHMMDVQGST QDSAIKDFVL KYALPLVGHR

    KVSNDAKRYT RRPLVVVYYS VDFSFDYRAA TQFWRSKVLE VAKDFPEYTF

    AIADEEDYAG EVKDLGLSES GEDVNAAILD ESGKKFAMEP EEFDSDTLRE

    FVTAFKKGKL KPVIKSQPVP KNNKGPVKVV VGKTFDSIVM DPKKDVLIEF

    YAPWCGHCKQ LEPVYNSLAK KYKGQKGLVI AKMDATANDV PSDRYKVEGF

    PTIYFAPSGD KKNPVKFEGG DRDLEHLSKF IEEHATKLSR TKEEL

  • UCSC uc003wff.3; human.;



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