Human PHB ELISA Kit

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  • Alternative name

    Human HEL-215 ELISA Kit;Human HEL-S-54e ELISA Kit;Human PHB1 ELISA Kit;Human prohibitin ELISA Kit;Human epididymis luminal protein 215 ELISA Kit;Human epididymis secretory sperm binding protein Li 54e ELISA Kit;

  • Catalog
    E031034
  • species
    Human
  • GenePHB
  • Standard CurveHuman PHB ELISA Kit
  • Other Species Mouse Phb ELISA KitBovine PHB ELISA KitChicken PHB ELISA KitRat Phb ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman PHB ELISA Kit allows for the in vitro quantitative determination of PHB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Intended Uses: This PHB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PHB. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: PHB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PHB antibody and an PHB-HRP conjugate. The assay sample and buffer are incubated together with PHB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PHB concentration since PHB from samples and PHB-HRP conjugate compete for the anti-PHB antibody binding site. Since the number of sites is limited, as more sites are occupied by PHB from the sample, fewer sites are left to bind PHB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PHB concentration in each sample is interpolated from this standard curve.
  • Human Prohibitin Protein information
  • Uniprot ID PHB_HUMAN
  • Uniprot AC P35232; B4DY47; Q4VBQ0;
  • UniGene Hs.514303;
  • GeneID 5245
  • KEGG hsa:5245;
  • Human Prohibitin Protein SEQUENCE
  • SEQUENCE 272 AA; 29804 MW; 915494273E342C76 CRC64;

    MAAKVFESIG KFGLALAVAG GVVNSALYNV DAGHRAVIFD RFRGVQDIVV

    GEGTHFLIPW VQKPIIFDCR SRPRNVPVIT GSKDLQNVNI TLRILFRPVA

    SQLPRIFTSI GEDYDERVLP SITTEILKSV VARFDAGELI TQRELVSRQV

    SDDLTERAAT FGLILDDVSL THLTFGKEFT EAVEAKQVAQ QEAERARFVV

    EKAEQQKKAA IISAEGDSKA AELIANSLAT AGDGLIELRK LEAAEDIAYQ

    LSRSRNITYL PAGQSVLLQL PQ

  • UCSC uc002iox.3; human. [P35232-1];



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