Human SERPINC1 ELISA Kit

Alternative name
Human ATIII ELISA Kit;Human Serpin C1 ELISA Kit;Human AT3 ELISA Kit;Human AT3D ELISA Kit;Human THPH7 ELISA Kit;Human serpin family C member 1 ELISA Kit;Human antithrombin-III ELISA Kit;Human serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1 ELISA Kit;Human serpin peptidase inhibitor clade C member 1 ELISA Kit;Human serpin peptidase inhibitor, clade C (antithrombin), member 1 ELISA Kit;
Catalog
E003141
species
Human
GeneSERPINC1
Standard Curve
Other Species Mouse Serpinc1 ELISA Kit Bovine SERPINC1 ELISA Kit Chicken SERPINC1 ELISA Kit Sheep SERPINC1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of AT III. No significant cross-reactivity or interference between AT III and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AT III and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman SERPINC1 ELISA Kit allows for the in vitro quantitative determination of SERPINC1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCardiovascular
Product Description
specificalPrinciple of the assay: AT III ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AT III antibody and an AT III-HRP conjugate. The assay sample and buffer are incubated together with AT III-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AT III concentration since AT III from samples and AT III-HRP conjugate compete for the anti-AT III antibody binding site. Since the number of sites is limited, as more sites are occupied by AT III from the sample, fewer sites are left to bind AT III-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AT III concentration in each sample is interpolated from this standard curve.
Human Antithrombin-III Protein information
Uniprot ID ANT3_HUMAN
Uniprot AC Q9UC78;
UniGene Hs.75599;
GeneID 462
KEGG hsa:462;
Human Antithrombin-III Protein SEQUENCE
SEQUENCE 464 AA; 52602 MW; 9A4E324F00683D9D CRC64;
MYSNVIGTVT SGKRKVYLLS LLLIGFWDCV TCHGSPVDIC TAKPRDIPMN
PMCIYRSPEK KATEDEGSEQ KIPEATNRRV WELSKANSRF ATTFYQHLAD
SKNDNDNIFL SPLSISTAFA MTKLGACNDT LQQLMEVFKF DTISEKTSDQ
IHFFFAKLNC RLYRKANKSS KLVSANRLFG DKSLTFNETY QDISELVYGA
KLQPLDFKEN AEQSRAAINK WVSNKTEGRI TDVIPSEAIN ELTVLVLVNT
IYFKGLWKSK FSPENTRKEL FYKADGESCS ASMMYQEGKF RYRRVAEGTQ
VLELPFKGDD ITMVLILPKP EKSLAKVEKE LTPEVLQEWL DELEEMMLVV
HMPRFRIEDG FSLKEQLQDM GLVDLFSPEK SKLPGIVAEG RDDLYVSDAF
HKAFLEVNEE GSEAAASTAV VIAGRSLNPN RVTFKANRPF LVFIREVPLN
TIIFMGRVAN PCVK
UCSC uc001gjt.4; human.;

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Human SERPINC1 ELISA Kit