Human ICOSLG ELISA Kit

Alternative name
Human B7 homolog 2 ELISA Kit;Human B7-H2 ELISA Kit;Human B7-like protein Gl50 ELISA Kit;Human B7-related protein 1 ELISA Kit;Human B7RP-1 ELISA Kit;Human B7H2 ELISA Kit;Human B7RP1 ELISA Kit;Human ICOSL ELISA Kit;Human KIAA0653 ELISA Kit;Human CD275 ELISA Kit;Human GL50 ELISA Kit;Human ICOS-L ELISA Kit;Human LICOS ELISA Kit;Human inducible T-cell costimulator ligand ELISA Kit;Human ICOS ligand ELISA Kit;Human B7 homologue 2 ELISA Kit;Human inducible T-cell co-stimulator ligand ELISA Kit;Human transmembrane protein B7-H2 ICOS ligand ELISA Kit;
Catalog
E019719
species
Human
GeneICOSLG
Standard Curve
Other Species Mouse Icoslg ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Inducible T-Cell Co Stimulator Ligand (ICOSLG). No significant cross-reactivity or interference between Inducible T-Cell Co Stimulator Ligand (ICOSLG) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.063ng/mL.
Intended UseHuman ICOSLG ELISA Kit allows for the in vitro quantitative determination of ICOSLG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inducible T-Cell Co Stimulator Ligand (ICOSLG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inducible T-Cell Co Stimulator Ligand (ICOSLG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inducible T-Cell Co Stimulator Ligand (ICOSLG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inducible T-Cell Co Stimulator Ligand (ICOSLG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human ICOS ligand Protein information
Uniprot ID ICOSL_HUMAN
Uniprot AC O75144; A8MUZ1; Q9HD18; Q9NRQ1;
UniGene Hs.14155;
GeneID 23308
KEGG hsa:23308;
Human ICOS ligand Protein SEQUENCE
SEQUENCE 302 AA; 33349 MW; 647934E21B55E34A CRC64;
MRLGSPGLLF LLFSSLRADT QEKEVRAMVG SDVELSCACP EGSRFDLNDV
YVYWQTSESK TVVTYHIPQN SSLENVDSRY RNRALMSPAG MLRGDFSLRL
FNVTPQDEQK FHCLVLSQSL GFQEVLSVEV TLHVAANFSV PVVSAPHSPS
QDELTFTCTS INGYPRPNVY WINKTDNSLL DQALQNDTVF LNMRGLYDVV
SVLRIARTPS VNIGCCIENV LLQQNLTVGS QTGNDIGERD KITENPVSTG
EKNAATWSIL AVLCLLVVVA VAIGWVCRDR CLQHSYAGAW AVSPETELTG
HV
UCSC uc002zee.5; human. [O75144-1];

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