Human PRCP ELISA Kit

Alternative name
Human angiotensinase C ELISA Kit;Human lysosomal carboxypeptidase C ELISA Kit;Human proline carboxypeptidase ELISA Kit;Human prolylcarboxypeptidase ELISA Kit;Human PRCP ELISA Kit;Human PCP ELISA Kit;Human HUMPCP ELISA Kit;Human lysosomal Pro-X carboxypeptidase ELISA Kit;
Catalog
E031170
species
Human
GenePRCP
SpecificityThis assay has high sensitivity and excellent specificity for detection of PRCP. No significant cross-reactivity or interference between PRCP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PRCP and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman PRCP ELISA Kit allows for the in vitro quantitative determination of PRCP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the assay: PRCP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PRCP antibody and an PRCP-HRP conjugate. The assay sample and buffer are incubated together with PRCP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRCP concentration since PRCP from samples and PRCP-HRP conjugate compete for the anti-PRCP antibody binding site. Since the number of sites is limited, as more sites are occupied by PRCP from the sample, fewer sites are left to bind PRCP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRCP concentration in each sample is interpolated from this standard curve.
Human Lysosomal Pro-X carboxypeptidase Protein information
Uniprot ID PCP_HUMAN
Uniprot AC P42785; A8MU24; B2R7B7; B3KRK5; B5BU34;
UniGene Hs.523936; Hs.733148;
GeneID 5547
KEGG hsa:5547;
Human Lysosomal Pro-X carboxypeptidase Protein SEQUENCE
SEQUENCE 496 AA; 55800 MW; FC5DD570D3D3AB4F CRC64;
MGRRALLLLL LSFLAPWATI ALRPALRALG SLHLPTNPTS LPAVAKNYSV
LYFQQKVDHF GFNTVKTFNQ RYLVADKYWK KNGGSILFYT GNEGDIIWFC
NNTGFMWDVA EELKAMLVFA EHRYYGESLP FGDNSFKDSR HLNFLTSEQA
LADFAELIKH LKRTIPGAEN QPVIAIGGSY GGMLAAWFRM KYPHMVVGAL
AASAPIWQFE DLVPCGVFMK IVTTDFRKSG PHCSESIHRS WDAINRLSNT
GSGLQWLTGA LHLCSPLTSQ DIQHLKDWIS ETWVNLAMVD YPYASNFLQP
LPAWPIKVVC QYLKNPNVSD SLLLQNIFQA LNVYYNYSGQ VKCLNISETA
TSSLGTLGWS YQACTEVVMP FCTNGVDDMF EPHSWNLKEL SDDCFQQWGV
RPRPSWITTM YGGKNISSHT NIVFSNGELD PWSGGGVTKD ITDTLVAVTI
SEGAHHLDLR TKNALDPMSV LLARSLEVRH MKNWIRDFYD SAGKQH
UCSC uc001ozr.4; human. [P42785-1];

Category

Human PRCP ELISA Kit

Human angiotensinase C ELISA Kit;Human lysosomal carboxypeptidase C ELISA Kit;Human proline carboxypeptidase ELISA Kit;Human prolylcarboxypeptidase ELISA Kit;Human PRCP ELISA Kit;Human PCP ELISA Kit;Human HUMPCP ELISA Kit;Human lysosomal Pro-X carboxypeptidase ELISA Kit;