Human FABP4 ELISA Kit

Alternative name
Human adipocyte lipid-binding protein ELISA Kit;Human ALBP ELISA Kit;Human adipocyte-type fatty acid-binding protein ELISA Kit;Human A-FABP ELISA Kit;Human AFABP ELISA Kit;Human Fatty acid-binding protein 4 ELISA Kit;Human HEL-S-104 ELISA Kit;Human aP2 ELISA Kit;Human fatty acid binding protein 4 ELISA Kit;Human fatty acid-binding protein, adipocyte ELISA Kit;Human adipocyte fatty acid binding protein ELISA Kit;Human epididymis secretory protein Li 104 ELISA Kit;Human fatty acid binding protein 4, adipocyte ELISA Kit;
Catalog
E014038
species
Human
GeneFABP4
SpecificityNatural and recombinant Human FABP4
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity Intra-assay Precision Intra-Assay Precision (Precision within an assay) Three samples of kn
Intended UseHuman FABP4 ELISA Kit allows for the in vitro quantitative determination of FABP4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: Human FABP4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FABP4 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: M1-A132) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FABP4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human FABP4 amount of sample captured in plate. Background/Introduction: FABP4, also known as aP2 (adipocyte Protein 2), encodes the fatty acid binding protein found in adipocytes. The FABP4 gene is mapped to 8q21. FABP4 functions as a cytosolic lipid chaperone in macrophages and is involved in regulating macrophage endoplasmic reticulum (ER) stress. Aditionally, FABP4 protein is expressed in HBEs and is strongly upregulated by both IL4 and IL13 and downregulated by IFN-gamma, suggesting a role in allergic airway inflammation.
Human Fatty acid-binding protein, adipocyte Protein information
Uniprot ID FABP4_HUMAN
Uniprot AC P15090; Q6IBA1;
UniGene Hs.391561;
GeneID 2167
KEGG hsa:2167;
Human Fatty acid-binding protein, adipocyte Protein SEQUENCE
SEQUENCE 132 AA; 14719 MW; 819D788FF4BF7235 CRC64;
MCDAFVGTWK LVSSENFDDY MKEVGVGFAT RKVAGMAKPN MIISVNGDVI
TIKSESTFKN TEISFILGQE FDEVTADDRK VKSTITLDGG VLVHVQKWDG
KSTTIKRKRE DDKLVVECVM KGVTSTRVYE RA
UCSC uc003ycd.3; human.;

Human FABP4 ELISA Kit

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