Human PLSCR1 ELISA Kit

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  • Alternative name

    Human PL scramblase 1 ELISA Kit;Human ca(2+)-dependent phospholipid scramblase 1 ELISA Kit;Human erythrocyte phospholipid scramblase ELISA Kit;Human MMTRA1B ELISA Kit;Human phospholipid scramblase 1 ELISA Kit;

  • Catalog
    E029250
  • species
    Human
  • GenePLSCR1
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman PLSCR1 ELISA Kit allows for the in vitro quantitative determination of PLSCR1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the Assay: PLSCR1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PLSCR1. Standards or samples are then added to the microtiter plate wells and PLSCR1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PLSCR1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PLSCR1 are added to each well to "sandwich" the PLSCR1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PLSCR1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLSCR1 concentration in each sample is interpolated from this standard curve.
  • Human Phospholipid scramblase 1 Protein information
  • Uniprot ID PLS1_HUMAN
  • Uniprot AC O15162; B2R8H8; B4DTE8;
  • UniGene Hs.130759;
  • GeneID 5359
  • KEGG hsa:5359;
  • Human Phospholipid scramblase 1 Protein SEQUENCE
  • SEQUENCE 318 AA; 35049 MW; 9860744DEC40616E CRC64;

    MDKQNSQMNA SHPETNLPVG YPPQYPPTAF QGPPGYSGYP GPQVSYPPPP

    AGHSGPGPAG FPVPNQPVYN QPVYNQPVGA AGVPWMPAPQ PPLNCPPGLE

    YLSQIDQILI HQQIELLEVL TGFETNNKYE IKNSFGQRVY FAAEDTDCCT

    RNCCGPSRPF TLRIIDNMGQ EVITLERPLR CSSCCCPCCL QEIEIQAPPG

    VPIGYVIQTW HPCLPKFTIQ NEKREDVLKI SGPCVVCSCC GDVDFEIKSL

    DEQCVVGKIS KHWTGILREA FTDADNFGIQ FPLDLDVKMK AVMIGACFLI

    DFMFFESTGS QEQKSGVW

  • UCSC uc003evx.5; human. [O15162-1];



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