Human PER1 ELISA Kit

Alternative name
Human hPER1 ELISA Kit;Human circadian clock protein PERIOD 1 ELISA Kit;Human circadian pacemaker protein RIGUI ELISA Kit;Human KIAA0482 ELISA Kit;Human PER ELISA Kit;Human RIGUI ELISA Kit;Human hPER ELISA Kit;Human period circadian clock 1 ELISA Kit;Human period circadian protein homolog 1 ELISA Kit;Human Period, drosophila, homolog of ELISA Kit;Human period homolog 1 ELISA Kit;
Catalog
E028646
species
Human
GenePER1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Period Circadian Protein 1 (PER1). No significant cross-reactivity or interference between Period Circadian Protein 1 (PER1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.122ng/mL.
Intended UseHuman PER1 ELISA Kit allows for the in vitro quantitative determination of PER1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Period Circadian Protein 1 (PER1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Period Circadian Protein 1 (PER1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Period Circadian Protein 1 (PER1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Period Circadian Protein 1 (PER1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Period circadian protein homolog 1 Protein information
Uniprot ID PER1_HUMAN
Uniprot AC VDCGSSTQ DPGHPDDPLF SELDGLGLEP MEEGGGEQGS SGGGSGEGEG CEEAQGGAKA
UniGene Hs.445534;
GeneID 5187
KEGG hsa:5187;
Human Period circadian protein homolog 1 Protein SEQUENCE
SEQUENCE 1290 AA; 136212 MW; 60B844468EEF4D1B CRC64;
MSGPLEGADG GGDPRPGESF CPGGVPSPGP PQHRPCPGPS LADDTDANSN
GSSGNESNGH ESRGASQRSS HSSSSGNGKD SALLETTESS KSTNSQSPSP
PSSSIAYSLL SASSEQDNPS TSGCSSEQSA RARTQKELMT ALRELKLRLP
PERRGKGRSG TLATLQYALA CVKQVQANQE YYQQWSLEEG EPCSMDMSTY
TLEELEHITS EYTLQNQDTF SVAVSFLTGR IVYISEQAAV LLRCKRDVFR
GTRFSELLAP QDVGVFYGST APSRLPTWGT GASAGSGLRD FTQEKSVFCR
IRGGPDRDPG PRYQPFRLTP YVTKIRVSDG APAQPCCLLI AERIHSGYEA
PRIPPDKRIF TTRHTPSCLF QDVDERAAPL LGYLPQDLLG APVLLFLHPE
DRPLMLAIHK KILQLAGQPF DHSPIRFCAR NGEYVTMDTS WAGFVHPWSR
KVAFVLGRHK VRTAPLNEDV FTPPAPSPAP SLDTDIQELS EQIHRLLLQP
VHSPSPTGLC GVGAVTSPGP LHSPGSSSDS NGGDAEGPGP PAPVTFQQIC
KDVHLVKHQG QQLFIESRAR PQSRPRLPAT GTFKAKALPC QSPDPELEAG
SAPVQAPLAL VPEEAERKEA SSCSYQQINC LDSILRYLES CNLPSTTKRK
CASSSSYTTS SASDDDRQRT GPVSVGTKKD PPSAALSGEG ATPRKEPVVG
GTLSPLALAN KAESVVSVTS QCSFSSTIVH VGDKKPPESD IIMMEDLPGL
APGPAPSPAP SPTVAPDPAP DAYRPVGLTK AVLSLHTQKE EQAFLSRFRD
LGRLRGLDSS STAPSALGER GCHHGPAPPS RRHHCRSKAK RSRHHQNPRA
EAPCYVSHPS PVPPSTPWPT PPATTPFPAV VQPYPLPVFS PRGGPQPLPP
APTSVPPAAF PAPLVTPMVA LVLPNYLFPT PSSYPYGALQ TPAEGPPTPA
SHSPSPSLPA LAPSPPHRPD SPLFNSRCSS PLQLNLLQLE ELPRAEGAAV
AGGPGSSAGP PPPSAEAAEP EARLAEVTES SNQDALSGSS DLLELLLQED
SRSGTGSAAS GSLGSGLGSG SGSGSHEGGS TSASITRSSQ SSHTSKYFGS
IDSSEAEAGA ARGGAEPGDQ VIKYVLQDPI WLLMANADQR VMMTYQVPSR
DMTSVLKQDR ERLRAMQKQQ PRFSEDQRRE LGAVHSWVRK GQLPRALDVM
ACVDCGSSTQ DPGHPDDPLF SELDGLGLEP MEEGGGEQGS SGGGSGEGEG
CEEAQGGAKA SSSQDLAMEE EEEGRSSSSP ALPTAGNCTS
UCSC uc002gkd.4; human. [O15534-1];

Category

Human PER1 ELISA Kit