Human ODC1 ELISA Kit

Alternative name
Human ODC ELISA Kit;Human ornithine decarboxylase 1 ELISA Kit;Human ornithine decarboxylase ELISA Kit;
Catalog
E027791
species
Human
GeneODC1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.188ng/ml
Intended UseHuman ODC1 ELISA Kit allows for the in vitro quantitative determination of ODC1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ODC ntibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ODC antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ODC amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ODC can be calculated.
Human Ornithine decarboxylase Protein information
Uniprot ID DCOR_HUMAN
Uniprot AC P11926; Q53TU3; Q6LDS9;
UniGene Hs.467701;
GeneID 4953
KEGG hsa:4953;
Human Ornithine decarboxylase Protein SEQUENCE
SEQUENCE 461 AA; 51148 MW; 8CCB88CE80E823C5 CRC64;
MNNFGNEEFD CHFLDEGFTA KDILDQKINE VSSSDDKDAF YVADLGDILK
KHLRWLKALP RVTPFYAVKC NDSKAIVKTL AATGTGFDCA SKTEIQLVQS
LGVPPERIIY ANPCKQVSQI KYAANNGVQM MTFDSEVELM KVARAHPKAK
LVLRIATDDS KAVCRLSVKF GATLRTSRLL LERAKELNID VVGVSFHVGS
GCTDPETFVQ AISDARCVFD MGAEVGFSMY LLDIGGGFPG SEDVKLKFEE
ITGVINPALD KYFPSDSGVR IIAEPGRYYV ASAFTLAVNI IAKKIVLKEQ
TGSDDEDESS EQTFMYYVND GVYGSFNCIL YDHAHVKPLL QKRPKPDEKY
YSSSIWGPTC DGLDRIVERC DLPEMHVGDW MLFENMGAYT VAAASTFNGF
QRPTIYYVMS GPAWQLMQQF QNPDFPPEVE EQDASTLPVS CAWESGMKRH
RAACASASIN V
UCSC uc002rao.3; human.;

Category

Human ODC1 ELISA Kit

Human ODC ELISA Kit;Human ornithine decarboxylase 1 ELISA Kit;Human ornithine decarboxylase ELISA Kit;