Human IER5 ELISA Kit

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  • Alternative name

    Human SBBI48 ELISA Kit;Human immediate early response 5 ELISA Kit;Human immediate early response gene 5 protein ELISA Kit;

  • Catalog
    E019541
  • species
    Human
  • GeneIER5
  • Other Species Mouse Ier5 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human IER5. No significant cross-reactivity or interference between human IER5 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human IER5 is typically less than 3.9 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
  • Intended UseHuman IER5 ELISA Kit allows for the in vitro quantitative determination of IER5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IER5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IER5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IER5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IER5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Immediate early response gene 5 protein Protein information
  • Uniprot ID IER5_HUMAN
  • Uniprot AC Q5VY09; B2RBV3; Q8WY68; Q9NY49; Q9NZP9;
  • UniGene Hs.15725;
  • GeneID 51278
  • KEGG hsa:51278;
  • Human Immediate early response gene 5 protein Protein SEQUENCE
  • SEQUENCE 327 AA; 33704 MW; E841C5FAF6520A12 CRC64;

    MEFKLEAHRI VSISLGKIYN SRVQRGGIKL HKNLLVSLVL RSARQVYLSD

    PCPGLYLAGP AGTPAPPPQQ QPGEPAAGPP AGWGEPPPPA ARASWPETEP

    QPERSSVSDA PRVGDEVPVA TVTGVGDVFQ GGEADATEAA WSRVEGPRQA

    AAREAEGTAG GWGVFPEVSR AARRPCGCPL GGEDPPGTPA ATPRAACCCA

    PQPAEDEPPA PPAVCPRKRC AAGVGGGPAG CPAPGSTPLK KPRRNLEQPP

    SGGEDDDAEE METGNVANLI SIFGSSFSGL LRKSPGGGRE EEEGEESGPE

    AAEPGQICCD KPVLRDMNPW STAIVAF

  • UCSC uc001got.5; human.;



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