Human NXN ELISA Kit

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  • Alternative name

    Human NRX ELISA Kit;Human TRG-4 ELISA Kit;Human nucleoredoxin ELISA Kit;Human nucleoredoxin 1 ELISA Kit;

  • Catalog
    E027129
  • species
    Human
  • GeneNXN
  • Standard CurveHuman NXN ELISA Kit
  • Other Species Mouse Nxn ELISA KitBovine NXN ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Nucleoredoxin (NXN). No significant cross-reactivity or interference between Nucleoredoxin (NXN) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.063ng/mL.
  • Intended UseHuman NXN ELISA Kit allows for the in vitro quantitative determination of NXN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nucleoredoxin (NXN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nucleoredoxin (NXN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nucleoredoxin (NXN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nucleoredoxin (NXN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Nucleoredoxin Protein information
  • Uniprot ID NXN_HUMAN
  • Uniprot AC Q6DKJ4; B4DXQ0; D3DTH2; Q3SWW6; Q6P3U6; Q7L4C6; Q9H9Q1;
  • UniGene Hs.527989;
  • GeneID 64359
  • KEGG hsa:64359;
  • Human Nucleoredoxin Protein SEQUENCE
  • SEQUENCE 435 AA; 48392 MW; B67927F001BE4E5B CRC64;

    MSGFLEELLG EKLVTGGGEE VDVHSLGARG ISLLGLYFGC SLSAPCAQLS

    ASLAAFYGRL RGDAAAGPGP GAGAGAAAEP EPRRRLEIVF VSSDQDQRQW

    QDFVRDMPWL ALPYKEKHRK LKLWNKYRIS NIPSLIFLDA TTGKVVCRNG

    LLVIRDDPEG LEFPWGPKPF REVIAGPLLR NNGQSLESSS LEGSHVGVYF

    SAHWCPPCRS LTRVLVESYR KIKEAGQNFE IIFVSADRSE ESFKQYFSEM

    PWLAVPYTDE ARRSRLNRLY GIQGIPTLIM LDPQGEVITR QGRVEVLNDE

    DCREFPWHPK PVLELSDSNA AQLNEGPCLV LFVDSEDDGE SEAAKQLIQP

    IAEKIIAKYK AKEEEAPLLF FVAGEDDMTD SLRDYTNLPE AAPLLTILDM

    SARAKYVMDV EEITPAIVEA FVNDFLAEKL KPEPI

  • UCSC uc002fsa.4; human. [Q6DKJ4-1];


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